Identification of 7-Carboxymethylguanine in DNA from Pancreatic Acinar Cells Exposed to Azaserine

Joanne Zurlo; Thomas J. Curnhav; Raymond Hiley; Daniel S. Longnecker; Joanne Zurlo

Identification of 7-Carboxymethylguanine in DNA from Pancreatic Acinar Cells Exposed to Azaserine

Joanne Zurlo, Thomas J. Curnhav, Raymond Hiley, Daniel S. Longnecker, Joanne Zurlo

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Studies were undertaken to determine the identity of an azaserine:DNA adduct. The most probable adduct, 7-carbox-ymethylguanine, was synthesized. DNA isolated from pancreatic acinar cells treated in culture with [¹⁴C]azaserine was hydrolyzed under neutral conditions to liberate N-alkylated purines. The neutral hydrolysate was subjected to high-per-formance liquid chromatography along with the synthetic standard. One of the radioactive peaks from the treated DNA was found to cochromatograph with 7-carboxymethylguanine in three systems: reverse phase; anion exchange; and ion pair reverse phase. These results suggest that azaserine metabolism in acinar cells results in carboxymethylation of DNA, supporting previously proposed models of azaserine degradation.

Original language	English (US)
Pages (from-to)	1286-1288
Number of pages	3
Journal	Cancer Research
Volume	42
Issue number	4
State	Published - Apr 1 1982

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

@article{9b4c3ff8bc554789b19a227db7ba4483,

title = "Identification of 7-Carboxymethylguanine in DNA from Pancreatic Acinar Cells Exposed to Azaserine",

abstract = "Studies were undertaken to determine the identity of an azaserine:DNA adduct. The most probable adduct, 7-carbox-ymethylguanine, was synthesized. DNA isolated from pancreatic acinar cells treated in culture with [14C]azaserine was hydrolyzed under neutral conditions to liberate N-alkylated purines. The neutral hydrolysate was subjected to high-per-formance liquid chromatography along with the synthetic standard. One of the radioactive peaks from the treated DNA was found to cochromatograph with 7-carboxymethylguanine in three systems: reverse phase; anion exchange; and ion pair reverse phase. These results suggest that azaserine metabolism in acinar cells results in carboxymethylation of DNA, supporting previously proposed models of azaserine degradation.",

author = "Joanne Zurlo and Curnhav, {Thomas J.} and Raymond Hiley and Longnecker, {Daniel S.} and Joanne Zurlo",

year = "1982",

month = apr,

day = "1",

language = "English (US)",

volume = "42",

pages = "1286--1288",

journal = "Cancer Research",

issn = "0008-5472",

publisher = "American Association for Cancer Research Inc.",

number = "4",

}

TY - JOUR

T1 - Identification of 7-Carboxymethylguanine in DNA from Pancreatic Acinar Cells Exposed to Azaserine

AU - Zurlo, Joanne

AU - Curnhav, Thomas J.

AU - Hiley, Raymond

AU - Longnecker, Daniel S.

AU - Zurlo, Joanne

PY - 1982/4/1

Y1 - 1982/4/1

N2 - Studies were undertaken to determine the identity of an azaserine:DNA adduct. The most probable adduct, 7-carbox-ymethylguanine, was synthesized. DNA isolated from pancreatic acinar cells treated in culture with [14C]azaserine was hydrolyzed under neutral conditions to liberate N-alkylated purines. The neutral hydrolysate was subjected to high-per-formance liquid chromatography along with the synthetic standard. One of the radioactive peaks from the treated DNA was found to cochromatograph with 7-carboxymethylguanine in three systems: reverse phase; anion exchange; and ion pair reverse phase. These results suggest that azaserine metabolism in acinar cells results in carboxymethylation of DNA, supporting previously proposed models of azaserine degradation.

AB - Studies were undertaken to determine the identity of an azaserine:DNA adduct. The most probable adduct, 7-carbox-ymethylguanine, was synthesized. DNA isolated from pancreatic acinar cells treated in culture with [14C]azaserine was hydrolyzed under neutral conditions to liberate N-alkylated purines. The neutral hydrolysate was subjected to high-per-formance liquid chromatography along with the synthetic standard. One of the radioactive peaks from the treated DNA was found to cochromatograph with 7-carboxymethylguanine in three systems: reverse phase; anion exchange; and ion pair reverse phase. These results suggest that azaserine metabolism in acinar cells results in carboxymethylation of DNA, supporting previously proposed models of azaserine degradation.

UR - http://www.scopus.com/inward/record.url?scp=0020048127&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020048127&partnerID=8YFLogxK

M3 - Article

C2 - 7060007

AN - SCOPUS:0020048127

SN - 0008-5472

VL - 42

SP - 1286

EP - 1288

JO - Cancer Research

JF - Cancer Research

IS - 4

ER -

Identification of 7-Carboxymethylguanine in DNA from Pancreatic Acinar Cells Exposed to Azaserine

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this