Identification of 3'UTR region implicated in tau mRNA stabilization in neuronal cells

Stella Aronov, Ruth Marx, Irith Ginzburg

Research output: Contribution to journalArticlepeer-review


Tau, a neuronal microtuble-associated protein (MAP) plays an important role in the formation and maintenance of neuronal polarity. Tau mRNA is a stable message and exhibits a relatively long half-life in neuronal cells. The regulation of mRNA stability is a crucial determinant in controlling mRNA steady-state levels in neuronal cells and thereby influences gene expression. The half-lives of specific mRNAs may be dependent on specific sequences located at their 3'untranslated region (UTR), which in turn, may be recognized by tissue-specific proteins. To identify the sequence elements involved in tau mRNA stabilization, selected regions of the 3'UTR were subcloned downstream to c-fos reporter mRNA or to the coding region of the tau mRNA. Using stably transfected neuronal cells, we have demonstrated that a fragment of 240 bp (H fragment) located in the 3'UTR can stabilize c-fos and tau mRNAs. Analysis of stably transfected cells indicated that the transfected tau mRNAs are associated with the microtubules of neuronal cells, suggesting that this association may play a role in tau mRNA stabilization. This step may be a prerequisite in the multistep process leading to the subcellular localization of tau mRNA in neuronal cells.

Original languageEnglish (US)
Pages (from-to)131-145
Number of pages15
JournalJournal of Molecular Neuroscience
Issue number2
StatePublished - 1999
Externally publishedYes


  • 3'untranslated region
  • LAN-1 cells
  • Message stabilization
  • Microtubules
  • PC12 cells
  • Tau mRNA

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience


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