TY - JOUR
T1 - Identification and characterization of the highly polymorphic locus D14S739 in the Han Chinese population
AU - Shao, Chengchen
AU - Zhang, Yaqi
AU - Zhou, Yueqin
AU - Zhu, Wei
AU - Xu, Hongmei
AU - Liu, Zhiping
AU - Tang, Qiqun
AU - Shen, Yiwen
AU - Xie, Jianhui
PY - 2015
Y1 - 2015
N2 - Aim: To systemically select and evaluate short tandem repeats (STRs) on the chromosome 14 and obtain new STR loci as expanded genotyping markers for forensic application. Methods: STRs on the chromosome 14 were filtered from Tandem Repeats Database and further selected based on their positions on the chromosome, repeat patterns of the core sequences, sequence homology of the flanking regions, and suitability of flanking regions in primer design. The STR locus with the highest heterozygosity and polymorphism information content (PIC) was selected for further analysis of genetic polymorphism, forensic parameters, and the core sequence. Results: Among 26 STR loci selected as candidates, D14S739 had the highest heterozygosity (0.8691) and PIC (0.8432), and showed no deviation from the Hardy-Weinberg equilibrium. 14 alleles were observed, ranging in size from 21 to 34 tetranucleotide units in the core region of (GATA)9-18 (GACA)7-12 GACG (GACA)2 GATA. Paternity testing showed no mutations. Conclusion: D14S739 is a highly informative STR locus and could be a suitable genetic marker for forensic applications in the Han Chinese population.
AB - Aim: To systemically select and evaluate short tandem repeats (STRs) on the chromosome 14 and obtain new STR loci as expanded genotyping markers for forensic application. Methods: STRs on the chromosome 14 were filtered from Tandem Repeats Database and further selected based on their positions on the chromosome, repeat patterns of the core sequences, sequence homology of the flanking regions, and suitability of flanking regions in primer design. The STR locus with the highest heterozygosity and polymorphism information content (PIC) was selected for further analysis of genetic polymorphism, forensic parameters, and the core sequence. Results: Among 26 STR loci selected as candidates, D14S739 had the highest heterozygosity (0.8691) and PIC (0.8432), and showed no deviation from the Hardy-Weinberg equilibrium. 14 alleles were observed, ranging in size from 21 to 34 tetranucleotide units in the core region of (GATA)9-18 (GACA)7-12 GACG (GACA)2 GATA. Paternity testing showed no mutations. Conclusion: D14S739 is a highly informative STR locus and could be a suitable genetic marker for forensic applications in the Han Chinese population.
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U2 - 10.3325/cmj.2015.56.482
DO - 10.3325/cmj.2015.56.482
M3 - Article
C2 - 26526885
AN - SCOPUS:84948756066
SN - 0353-9504
VL - 56
SP - 482
EP - 489
JO - Croatian Medical Journal
JF - Croatian Medical Journal
IS - 5
ER -