Identification and characterization of citrulline-modified brain proteins by combining HCD and CID fragmentation

Zhicheng Jin, Zongming Fu, Jun Yang, Juan Troncosco, Allen D. Everett, Jennifer E. Van Eyk

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Citrullination is a protein PTM of arginine residues catalyzed by peptidylarginine deiminase. Protein citrullination has been detected in the CNS and associated with a number of neurological diseases. However, identifying citrullinated proteins from complex mixtures and pinpointing citrullinated residues have been limited. Using RP LC and high-resolution MS, this study determined in vitro citrullination sites of glial fibrillary acid protein (GFAP), neurogranin (NRGN/RC3), and myelin basic protein (MBP) and in vivo sites in brain protein extract. Human GFAP has five endogenous citrullination sites, R30, R36, R270, R406, and R416, and MBP has 14 in vivo citrullination sites. Human NRGN/RC3 was found citrullinated at residue R68. The sequence of citrullinated peptides and citrullination sites were confirmed from peptides identified in trypsin, Lys-C, and Glu-C digests. The relative ratio of citrullination was estimated by simultaneous identification of citrullinated and unmodified peptides from Alzheimer's and control brain samples. The site occupancy of citrullination at the residue R68 of NRGN ranged from 1.6 to 9.5%. Compared to CID, higher-energy collisional dissociation (HCD) mainly produced protein backbone fragmentation for citrullinated peptides. CID-triggered HCD fragmentation is an optimal approach for the identification of citrullinated peptides in complex protein digests.

Original languageEnglish (US)
Pages (from-to)2682-2691
Number of pages10
Issue number17
StatePublished - Sep 2013


  • Biomedicine
  • Citrullination
  • GFAP
  • MBP
  • Mass spectrometry
  • NRGN

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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