TY - JOUR
T1 - Identification and Characterization of a Novel Inositol Hexakisphosphate Kinase
AU - Saiardi, Adolfo
AU - Nagata, Eiichiro
AU - Luo, Hongbo R.
AU - Snowman, Adele M.
AU - Snyder, Solomon H.
PY - 2001/10/19
Y1 - 2001/10/19
N2 - The inositol pyrophosphate disphosphoinositol pentakisphosphate (PP-InsP3/InsP7) is formed in mammals by two recently cloned inositol hexakiphosphate kinases, InsP6K1 and InsP 6K2 (Saiardi, A., Erdjument-Bromage, H., Snowman, A. M., Tempst, P., and Snyder, S. H. (1999) Curr. Biol. 9, 1323-1326). We now report the identification, cloning, and characterization of a third InsP7 forming enzyme designated InsP6K3. InsP6K3 displays 50 and 45% sequence identity to InsP6K1 and InsP6K2, respectively, with a smaller mass (46 kDa) and a more basic character than the other two enzymes. InsP6K3 is most enriched in the brain where its localization resembles InsP6K1 and InsP6K2. Intracellular disposition discriminates the three enzymes with InsP6K2 being exclusively nuclear, InsP6K3 predominating in the cytoplasm, and InsP6K1 displaying comparable nuclear and cytosolic densities.
AB - The inositol pyrophosphate disphosphoinositol pentakisphosphate (PP-InsP3/InsP7) is formed in mammals by two recently cloned inositol hexakiphosphate kinases, InsP6K1 and InsP 6K2 (Saiardi, A., Erdjument-Bromage, H., Snowman, A. M., Tempst, P., and Snyder, S. H. (1999) Curr. Biol. 9, 1323-1326). We now report the identification, cloning, and characterization of a third InsP7 forming enzyme designated InsP6K3. InsP6K3 displays 50 and 45% sequence identity to InsP6K1 and InsP6K2, respectively, with a smaller mass (46 kDa) and a more basic character than the other two enzymes. InsP6K3 is most enriched in the brain where its localization resembles InsP6K1 and InsP6K2. Intracellular disposition discriminates the three enzymes with InsP6K2 being exclusively nuclear, InsP6K3 predominating in the cytoplasm, and InsP6K1 displaying comparable nuclear and cytosolic densities.
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U2 - 10.1074/jbc.M106842200
DO - 10.1074/jbc.M106842200
M3 - Article
C2 - 11502751
AN - SCOPUS:0035914367
SN - 0021-9258
VL - 276
SP - 39179
EP - 39185
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 42
ER -