Hypoxia-ischemia produces focal disruption of glutamate receptors in developing brain

Faye S. Silverstein, Lynn Torke, John Barks, Michael V Johnston

Research output: Contribution to journalArticlepeer-review

60 Scopus citations


We examined the impact of a perinatal hypoxic-ischemic insult on the distribution of glutamate receptors in developing brain. We used a well characterized rodent model for perinatal hypoxic-ischemic encephalopathy, unilateral carotid artery occlusion followed by exposure to 8% oxygen for 2.5 h in 7-day-old rat pups. This preparation results in focal neuronal damage in striatum, hippocampus, and cortex ipsilateral to ligation. Alterations in the regional distribution of glutamate binding in the first 24 h after the insult were assessed with quantitative in vitro [3H]glutamate autoradiography. In lesioned animals, we found progressive selective reductions in [3H]glutamate binding in forebrain ipsilateral to ligation in regions destined for neuronal damage. The earliest and most prominent unilateral reductions in binding were noted in the dentate gyrus of hippocampus (-45 ± 9%, compared with contralateral hemisphere at 24 h). Acute reductions in specific glutamate binding appear to be a sensitive marker for hypoxic-ischemic neuronal damage in the immature brain. These observations suggest that neurons bearing glutamate receptors may be particularly susceptible to hypoxic-ischemic injury.

Original languageEnglish (US)
Pages (from-to)33-39
Number of pages7
JournalDevelopmental Brain Research
Issue number1
StatePublished - 1987
Externally publishedYes


  • Autoradiography
  • Dentate gyrus
  • Glutamic acid
  • Hypoxia-ischemia
  • Perinatal

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience


Dive into the research topics of 'Hypoxia-ischemia produces focal disruption of glutamate receptors in developing brain'. Together they form a unique fingerprint.

Cite this