@inbook{e79c77ef2133462ea6d7f1ae0027067d,
title = "Hypoxia-induced reporter genes with different half-lives",
abstract = "The utility of reporter genes has gained significant momentum over the last three decades. Reporter genes are used to understand the transcriptional activity of a gene both in vitro and in vivo, and in pathway analysis and drug screening for diseases involving protozoan parasites, and in anti-cancer drug developments. Here, using a human prostate cancer xenograft model (PC3), we describe a method to construct and validate hypoxia reporter genes with different half-lives. Using molecular biology and optical imaging techniques, we have validated the expression of long half-life enhanced green fluorescence protein (EGFP) expression and short half-life luciferase gene expression to report on the spatial and temporal evolution of hypoxia in vivo.",
keywords = "Bioluminescence, Hypoxia, Hypoxia response elements (HRE), Lentivirus, Luciferase assay, Reporter gene",
author = "Balaji Krishnamachary and Pierre Danhier and Samata Kakkad and Bharti, {Santosh K.} and Bhujwalla, {Zaver M.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2018.",
year = "2018",
doi = "10.1007/978-1-4939-7860-1_9",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "113--125",
booktitle = "Methods in Molecular Biology",
}