Hypoxia-induced reporter genes with different half-lives

Balaji Krishnamachary, Pierre Danhier, Samata Kakkad, Santosh K. Bharti, Zaver M. Bhujwalla

Research output: Chapter in Book/Report/Conference proceedingChapter


The utility of reporter genes has gained significant momentum over the last three decades. Reporter genes are used to understand the transcriptional activity of a gene both in vitro and in vivo, and in pathway analysis and drug screening for diseases involving protozoan parasites, and in anti-cancer drug developments. Here, using a human prostate cancer xenograft model (PC3), we describe a method to construct and validate hypoxia reporter genes with different half-lives. Using molecular biology and optical imaging techniques, we have validated the expression of long half-life enhanced green fluorescence protein (EGFP) expression and short half-life luciferase gene expression to report on the spatial and temporal evolution of hypoxia in vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages13
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Bioluminescence
  • Hypoxia
  • Hypoxia response elements (HRE)
  • Lentivirus
  • Luciferase assay
  • Reporter gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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