TY - JOUR
T1 - Hypomethylation of DNA from benign and malignant human colon neoplasms
AU - Goelz, Susan E.
AU - Vogelstein, Bert
AU - Hamilton, Stanley R.
AU - Feinberg, Andrew P.
PY - 1985
Y1 - 1985
N2 - The methylation state of DNA from human colon tissue displaying neoplastic growth was determined by means of restriction endonuclease analysis. When compared to DNA from adjacent normal tissue, DNA from both benign colon polyps and malignant carcinomas was substantially hypomethylated. With the use of probes for growth hormone, γ-globin, α-chorionic gonadotropin, and γ-crystallin, methylation changes were detected in all 23 neoplastic growths examined. Benign polyps were hypomethylated to a degree similar to that in malignant tissue. These results indicate that hypomethylation is a consistent biochemical characteristic of human colonic tumors and is an alteration in the DNA that precedes malignancy.
AB - The methylation state of DNA from human colon tissue displaying neoplastic growth was determined by means of restriction endonuclease analysis. When compared to DNA from adjacent normal tissue, DNA from both benign colon polyps and malignant carcinomas was substantially hypomethylated. With the use of probes for growth hormone, γ-globin, α-chorionic gonadotropin, and γ-crystallin, methylation changes were detected in all 23 neoplastic growths examined. Benign polyps were hypomethylated to a degree similar to that in malignant tissue. These results indicate that hypomethylation is a consistent biochemical characteristic of human colonic tumors and is an alteration in the DNA that precedes malignancy.
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U2 - 10.1126/science.2579435
DO - 10.1126/science.2579435
M3 - Article
C2 - 2579435
AN - SCOPUS:0021950948
SN - 0036-8075
VL - 228
SP - 187
EP - 190
JO - Science
JF - Science
IS - 4696
ER -