TY - JOUR
T1 - Hypermethylation of HLA-C may be an epigenetic marker in psoriasis
AU - Chen, Min
AU - Wang, Yan
AU - Yao, Xu
AU - Li, Chengrang
AU - Jiang, Mingjun
AU - Cui, Pangen
AU - Wang, Baoxi
PY - 2016/1/8
Y1 - 2016/1/8
N2 - Background: There are no published studies that describe the DNA methylation of human leukocyte antigen class I molecules in psoriasis despite the fact that their association to disease has been known for several decades. Objective: we investigated the methylation status of HLA-A, -B and -C loci in psoriatic epidermis. Methods: The DNA and RNA specimens were obtained from the involved and uninvoled epidermis of 56 patients with plaque psoriasis and 28 healthy persons as the control group. Methylation of HLA was examined by MSP and Bisulfite sequencing. HLA-C mRNA expression was examined by Q-PCR. The severity of disease was evaluated by psoriasis area and severity index (PASI). Results: In psoriatic lesions and psoriatic non-lesions, the percentage of promoter methylation for HLA-B was 3.57% (2/56) and 3.57% (2/56), while it was 14.28% (8/56) and 23.21% (13/56) for HLA-C, respectively. Methylation of HLA-A was not be detected in both psoriatic lesions and non-lesions. Methylation of HLA-A, -B and -C loci was not found in healthy controls. The frequency of promoter methylation for HLA-C in the psoriatic epidermis was significantly increased compared with healthy controls (p2 =1.465, p=0.167), which was further confirmed by using bisulfite sequencing (t=1.958, p=0.055). HLA-C methylation was not found in healthy controls. The mean methylation rate of HLA-C in psoriatic lesions is relative to PASI score (r=0.316, p=0.018). The mean methylation rate of HLA-C in psoriatic lesions and non-lesions of the patients (onset age ≤18 years) are higher than the other patients, respectively (t=6.884, p
AB - Background: There are no published studies that describe the DNA methylation of human leukocyte antigen class I molecules in psoriasis despite the fact that their association to disease has been known for several decades. Objective: we investigated the methylation status of HLA-A, -B and -C loci in psoriatic epidermis. Methods: The DNA and RNA specimens were obtained from the involved and uninvoled epidermis of 56 patients with plaque psoriasis and 28 healthy persons as the control group. Methylation of HLA was examined by MSP and Bisulfite sequencing. HLA-C mRNA expression was examined by Q-PCR. The severity of disease was evaluated by psoriasis area and severity index (PASI). Results: In psoriatic lesions and psoriatic non-lesions, the percentage of promoter methylation for HLA-B was 3.57% (2/56) and 3.57% (2/56), while it was 14.28% (8/56) and 23.21% (13/56) for HLA-C, respectively. Methylation of HLA-A was not be detected in both psoriatic lesions and non-lesions. Methylation of HLA-A, -B and -C loci was not found in healthy controls. The frequency of promoter methylation for HLA-C in the psoriatic epidermis was significantly increased compared with healthy controls (p2 =1.465, p=0.167), which was further confirmed by using bisulfite sequencing (t=1.958, p=0.055). HLA-C methylation was not found in healthy controls. The mean methylation rate of HLA-C in psoriatic lesions is relative to PASI score (r=0.316, p=0.018). The mean methylation rate of HLA-C in psoriatic lesions and non-lesions of the patients (onset age ≤18 years) are higher than the other patients, respectively (t=6.884, p
KW - Gene methylation
KW - HLA
KW - MRNA
KW - Psoriasis
UR - http://www.scopus.com/inward/record.url?scp=84964583067&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84964583067&partnerID=8YFLogxK
U2 - 10.1016/j.jdermsci.2016.04.003
DO - 10.1016/j.jdermsci.2016.04.003
M3 - Article
C2 - 27132688
AN - SCOPUS:84964583067
SN - 0923-1811
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
ER -