Hyperaccurate and Error-Prone Ribosomes Exploit Distinct Mechanisms during tRNA Selection

Hani S. Zaher, Rachel Green

Research output: Contribution to journalArticlepeer-review

66 Scopus citations


Escherichia coli strains displaying hyperaccurate (restrictive) and ribosomal ambiguity (ram) phenotypes have long been associated with alterations in rpsL and rpsD/rpsE, respectively. Crystallographic evidence shows the ribosomal proteins S12 and S4/S5 (corresponding to these genes) to be located in separate regions of the small ribosomal subunit that are important for domain closure thought to take place during tRNA selection. Mechanistically, the process of tRNA selection is separated into two distinct phases, initial selection and proofreading. Here, we explore the effects of mutations in rpsL and rpsD on these steps. Surprisingly, both restrictive and ram ribosomes primarily affect tRNA selection through alteration of the off rates of the near-cognate tRNA species but during distinct phases of the overall process (proofreading and initial selection, respectively). These observations suggest that closure interfaces (S12/h27/h44 versus S4/S5) in two distinct regions of the small ribosomal subunit function independently to promote high-fidelity tRNA selection.

Original languageEnglish (US)
Pages (from-to)110-120
Number of pages11
JournalMolecular cell
Issue number1
StatePublished - Jul 2010


  • RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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