TY - JOUR
T1 - Hyalocytes synthesize and secrete inhibitors of retinal pigment epithelial cell proliferation in vitro
AU - Lazarus, Howard S.
AU - Schoenfeld, Carl Ludwig
AU - Fekrat, Sharon
AU - Cohen, Steven
AU - Carol, Alicia
AU - Hageman, Gregory S.
AU - Hackett, Scan
AU - Chen, Ying Shan
AU - Vinores, Stanley A.
AU - Campochiaro, Peter A.
PY - 1996
Y1 - 1996
N2 - Background: Retinal pigment epithelial (RPE) cells that enter the vitreous in pathologic conditions, such as retinal detachment, may proliferate and contribute to the formation of epiretinal membranes. Objective: To study whether hyalocytes, endogenous vitreous cells, play a role in modulating the proliferation of RPE cells. Methods: Cell proliferation was measured by tritiated thymidine incorporation in density-arrested human RPE cells after incubation with media that had been conditioned by cultured bovine hyalocytes. Preliminary characterization of inhibitory activity in hyalocyte- conditioned medium was performed, including blocking experiments with a neutralizing antibody to transforming growth factor-β2 (TGF-β) and proliferation assays that used MV-1-Lu mink lung epithelial cells. Northern blots were done to assess hyalocyte expression of TGF-β messenger RNA. Results: Hyalocyte-conditioned medium inhibited tritiated thymidine incorporation in RPE cells and MV-1-Lu mink lung epithelial cells in the presence or absence of serum or protease inhibitors. A portion of the inhibitory activity was neutralized by an antibody directed against TGF-β. Northern blots of hyalocyte RNA demonstrated the presence of messenger RNA for TGF-β2. These data suggest that TGF-β is responsible for a portion of the inhibitory activity secreted by hyalocytes. Additional inhibitory activity is attributable to one or more low-molecular-weight molecules distinct from TGF-β. Conclusion: Hyalocyte-conditioned medium inhibits RPE cell proliferation in vitro through TGF-β and at least one other molecule. Production of these factors by hyalocytes in vivo could provide a deterrent for epiretinal membrane formation that may be perturbed under pathologic conditions.
AB - Background: Retinal pigment epithelial (RPE) cells that enter the vitreous in pathologic conditions, such as retinal detachment, may proliferate and contribute to the formation of epiretinal membranes. Objective: To study whether hyalocytes, endogenous vitreous cells, play a role in modulating the proliferation of RPE cells. Methods: Cell proliferation was measured by tritiated thymidine incorporation in density-arrested human RPE cells after incubation with media that had been conditioned by cultured bovine hyalocytes. Preliminary characterization of inhibitory activity in hyalocyte- conditioned medium was performed, including blocking experiments with a neutralizing antibody to transforming growth factor-β2 (TGF-β) and proliferation assays that used MV-1-Lu mink lung epithelial cells. Northern blots were done to assess hyalocyte expression of TGF-β messenger RNA. Results: Hyalocyte-conditioned medium inhibited tritiated thymidine incorporation in RPE cells and MV-1-Lu mink lung epithelial cells in the presence or absence of serum or protease inhibitors. A portion of the inhibitory activity was neutralized by an antibody directed against TGF-β. Northern blots of hyalocyte RNA demonstrated the presence of messenger RNA for TGF-β2. These data suggest that TGF-β is responsible for a portion of the inhibitory activity secreted by hyalocytes. Additional inhibitory activity is attributable to one or more low-molecular-weight molecules distinct from TGF-β. Conclusion: Hyalocyte-conditioned medium inhibits RPE cell proliferation in vitro through TGF-β and at least one other molecule. Production of these factors by hyalocytes in vivo could provide a deterrent for epiretinal membrane formation that may be perturbed under pathologic conditions.
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U2 - 10.1001/archopht.1996.01100130723015
DO - 10.1001/archopht.1996.01100130723015
M3 - Article
C2 - 8639087
AN - SCOPUS:8944262824
SN - 0003-9950
VL - 114
SP - 731
EP - 736
JO - Archives of ophthalmology
JF - Archives of ophthalmology
IS - 6
ER -