Human tumour clonogenicity in agar is improved by cell-free ascites

M. P. Uitendaal, H. A J M Hubers, J. G. McVie, H. M. Pinedo

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Replacement of enriched CMRL 1066 medium by cell-free ascites from tumour patients in the human tumour clonogenic assay described by Hamburger and Salmon (1977) increased plating efficiency for ovarian cancer cells by a median of 8-fold (range 0.4-1012 fold). In 40 experiments, 2 cases had a lower plating efficiency when cultured in cell-free ascites, 10 grew neither in standard medium nor in cell-free ascites and in 2 cases, growth was observed only in cell-free ascites. With standard medium, we observed 53% growth (> 5 colonies/dish) and 41% evaluable for chemosensitivity testing (> 30 colonies/dish). With cell-free ascites as culture medium, these figures were 71% and 63%, respectively. While under standard conditions the highest plating efficiency observed was 0.25%, in 21% of the experiments done with cell-free ascites a plating efficiency higher than 1% was reached. We conclude that cell-free ascites is able to stimulate proliferation of ovarian cancer cells in agar and that the use of it extends the applicability of the clonogenic assay.

Original languageEnglish (US)
Pages (from-to)55-59
Number of pages5
JournalBritish Journal of Cancer
Issue number1
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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