Human pluripotent stem cells: Prospects and challenges as a source of cardiomyocytes for in vitro modeling and cell-based cardiac repair

Matthew E. Hartman, Dao Fu Dai, Michael A. Laflamme

Research output: Contribution to journalReview articlepeer-review

Abstract

Human pluripotent stem cells (PSCs) represent an attractive source of cardiomyocytes with potential applications including disease modeling, drug discovery and safety screening, and novel cell-based cardiac therapies. Insights from embryology have contributed to the development of efficient, reliable methods capable of generating large quantities of human PSC-cardiomyocytes with cardiac purities ranging up to 90%. However, for human PSCs to meet their full potential, the field must identify methods to generate cardiomyocyte populations that are uniform in subtype (e.g. homogeneous ventricular cardiomyocytes) and have more mature structural and functional properties. For in vivo applications, cardiomyocyte production must be highly scalable and clinical grade, and we will need to overcome challenges including graft cell death, immune rejection, arrhythmogenesis, and tumorigenic potential. Here we discuss the types of human PSCs, commonly used methods to guide their differentiation into cardiomyocytes, the phenotype of the resultant cardiomyocytes, and the remaining obstacles to their successful translation.

Original languageEnglish (US)
Pages (from-to)3-17
Number of pages15
JournalAdvanced Drug Delivery Reviews
Volume96
DOIs
StatePublished - Jan 15 2016
Externally publishedYes

Keywords

  • Cardiac regeneration
  • Cardiac repair
  • Cardiovascular disease
  • Disease modeling
  • Drug discovery
  • Embryonic stem cells
  • Heart failure
  • Induced pluripotent stem cells

ASJC Scopus subject areas

  • Pharmaceutical Science

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