Human PCK1 Encoding Phosphoenolpyruvate Carboxykinase Is Located on Chromosome 20q13.2

Hua Yu; Rachel Thun; Settara Chandrasekharappa; J. M. Trent; Ji Zhang; Miriam H. Meisler

doi:10.1006/geno.1993.1040

Human PCK1 Encoding Phosphoenolpyruvate Carboxykinase Is Located on Chromosome 20q13.2

Hua Yu, Rachel Thun, Settara Chandrasekharappa, J. M. Trent, Ji Zhang, Miriam H. Meisler

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Cytoplasmic liver phosphoenolpyruvate carboxykinase (GTP)(PEPCK) catalyzes a rate-limiting step in gluconeogenesis. Primers derived from the rat liver PEPCK sequence were used to amplify a portion of the human liver cDNA and to screen a YAC library of human genomic DNA. The sequences of human and rat PEPCK cDNA differed at 16% of the nucleotides compared (45/291). Analysis of a human/rodent hybrid mapping panel demonstrated concordant segregation of PCK1 with chromosome 20. Fluorescence in situ hybridization with YAC DNA further localized PCK1 to subband 20q13.2.

Original language	English (US)
Pages (from-to)	219-221
Number of pages	3
Journal	Genomics
Volume	15
Issue number	1
DOIs	https://doi.org/10.1006/geno.1993.1040
State	Published - Jan 1993
Externally published	Yes

ASJC Scopus subject areas

Genetics

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abstract = "Cytoplasmic liver phosphoenolpyruvate carboxykinase (GTP)(PEPCK) catalyzes a rate-limiting step in gluconeogenesis. Primers derived from the rat liver PEPCK sequence were used to amplify a portion of the human liver cDNA and to screen a YAC library of human genomic DNA. The sequences of human and rat PEPCK cDNA differed at 16% of the nucleotides compared (45/291). Analysis of a human/rodent hybrid mapping panel demonstrated concordant segregation of PCK1 with chromosome 20. Fluorescence in situ hybridization with YAC DNA further localized PCK1 to subband 20q13.2.",

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year = "1993",

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AU - Thun, Rachel

AU - Chandrasekharappa, Settara

AU - Trent, J. M.

AU - Zhang, Ji

AU - Meisler, Miriam H.

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AB - Cytoplasmic liver phosphoenolpyruvate carboxykinase (GTP)(PEPCK) catalyzes a rate-limiting step in gluconeogenesis. Primers derived from the rat liver PEPCK sequence were used to amplify a portion of the human liver cDNA and to screen a YAC library of human genomic DNA. The sequences of human and rat PEPCK cDNA differed at 16% of the nucleotides compared (45/291). Analysis of a human/rodent hybrid mapping panel demonstrated concordant segregation of PCK1 with chromosome 20. Fluorescence in situ hybridization with YAC DNA further localized PCK1 to subband 20q13.2.

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Human PCK1 Encoding Phosphoenolpyruvate Carboxykinase Is Located on Chromosome 20q13.2

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