TY - JOUR
T1 - Human Microphysiological Models of Intestinal Tissue and Gut Microbiome
AU - Steinway, Steven N.
AU - Saleh, Jad
AU - Koo, Bon Kyoung
AU - Delacour, Delphine
AU - Kim, Deok Ho
N1 - Funding Information:
The authors would like to thank Dr. Mark Donowitz, from the Department of Medicine, Division of Gastroenterology and Hepatology at the Johns Hopkins School of Medicine for his insightful comments on the manuscript. Funding. This work was supported by grants from the Human Frontier Science Program (RGP0038/2018) (to D-HK and DD), the National Institute of Diabetes and Digestive and Kidney Diseases (T32 DK007632) (to SS and D-HK), and the CNRS Défi Biomimétisme (to DD), the Groupama Foundation – Research Prize for Rare Diseases 2017 (to DD), the LabEx “Who Am I?” #ANR-11-LABX-0071 and the Université de Paris IdEx #ANR-18-IDEX-0001 funded by the French Government through its “Investments for the Future” program (to DD).
Publisher Copyright:
© Copyright © 2020 Steinway, Saleh, Koo, Delacour and Kim.
PY - 2020/7/31
Y1 - 2020/7/31
N2 - The gastrointestinal (GI) tract is a complex system responsible for nutrient absorption, digestion, secretion, and elimination of waste products that also hosts immune surveillance, the intestinal microbiome, and interfaces with the nervous system. Traditional in vitro systems cannot harness the architectural and functional complexity of the GI tract. Recent advances in organoid engineering, microfluidic organs-on-a-chip technology, and microfabrication allows us to create better in vitro models of human organs/tissues. These micro-physiological systems could integrate the numerous cell types involved in GI development and physiology, including intestinal epithelium, endothelium (vascular), nerve cells, immune cells, and their interplay/cooperativity with the microbiome. In this review, we report recent progress in developing micro-physiological models of the GI systems. We also discuss how these models could be used to study normal intestinal physiology such as nutrient absorption, digestion, and secretion as well as GI infection, inflammation, cancer, and metabolism.
AB - The gastrointestinal (GI) tract is a complex system responsible for nutrient absorption, digestion, secretion, and elimination of waste products that also hosts immune surveillance, the intestinal microbiome, and interfaces with the nervous system. Traditional in vitro systems cannot harness the architectural and functional complexity of the GI tract. Recent advances in organoid engineering, microfluidic organs-on-a-chip technology, and microfabrication allows us to create better in vitro models of human organs/tissues. These micro-physiological systems could integrate the numerous cell types involved in GI development and physiology, including intestinal epithelium, endothelium (vascular), nerve cells, immune cells, and their interplay/cooperativity with the microbiome. In this review, we report recent progress in developing micro-physiological models of the GI systems. We also discuss how these models could be used to study normal intestinal physiology such as nutrient absorption, digestion, and secretion as well as GI infection, inflammation, cancer, and metabolism.
KW - gut-on-a-chip
KW - intestinal tissue
KW - microbiome
KW - microfluidics
KW - microphysiological model
KW - organ chip
KW - organoid
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U2 - 10.3389/fbioe.2020.00725
DO - 10.3389/fbioe.2020.00725
M3 - Review article
C2 - 32850690
AN - SCOPUS:85089429343
SN - 2296-4185
VL - 8
JO - Frontiers in Bioengineering and Biotechnology
JF - Frontiers in Bioengineering and Biotechnology
M1 - 725
ER -