@article{9bef621db1984523a88ca958ec92e058,
title = "Human enteroids/colonoids and intestinal organoids functionally recapitulate normal intestinal physiology and pathophysiology",
abstract = "Identification of Lgr5 as the intestinal stem cell markeras well as the growth factors necessary to replicate adult intestinal stem cell division has led to the establishment of the methods to generate {"}indefinite{"} ex vivo primary intestinal epithelial cultures, termed {"}mini-intestines.{"} Primary cultures developed from isolated intestinal crypts or stem cells (termed enteroids/colonoids) and from inducible pluripotent stem cells (termed intestinal organoids) are being applied to study human intestinal physiology and pathophysiology with great expectations for translational applications, including regenerative medicine. Here we discuss the physiologic properties of these cultures, their current use in understanding diarrhea-causing host-pathogen interactions, and potential future applications.",
author = "Zachos, {Nicholas C.} and Olga Kovbasnjuk and Jennifer Foulke-Abel and Julie In and Blutt, {Sarah E.} and {De Jonge}, {Hugo R.} and Estes, {Mary K.} and Mark Donowitz",
note = "Funding Information: This work was supported by National Institutes of Health Grants U18-TR000552, T32-DK007632, R01-DK026523, R01-DK061765, P01-DK072084, R01-AI080656, and P30-DK056338 (to M. K. E.) and P30-DK089502 (to M. D). This is the first article in the Thematic Minireview series {"}Modern Technologies for In-cell Biochemistry.{"} The authors declare that they have no conflicts of interest with the contents of this article. The content is solely the responsibility of the author and does not necessarily represent the official views of the National Institutes of Health. 1 Both authors contributed equally to this work. Publisher Copyright: {\textcopyright} 2016 by The American Society for Biochemistry and Molecular Biology, Inc.",
year = "2016",
month = feb,
day = "19",
doi = "10.1074/jbc.R114.635995",
language = "English (US)",
volume = "291",
pages = "3759--3766",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "8",
}