Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner

Fengjue Shu; Shuguang Guo; Yongjun Dang; Meiyan Qi; Guangjin Zhou; Zekun Guo; Ying Zhang; Chaoqun Wu; Shouyuan Zhao; Long Yu

doi:10.1023/A:1027317014159

Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner

Fengjue Shu
, Shuguang Guo
, Yongjun Dang
, Meiyan Qi
, Guangjin Zhou
, Zekun Guo
, Ying Zhang
, Chaoqun Wu
, Shouyuan Zhao
, Long Yu

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Human Aurora/Ip11-related kinase 2 (Aurora-B) is a key regulator of mitosis. Here human proteasome α-subunit C8 (HC8) was identified to interact with the Aurora-B by yeast two-hybrid screen. This finding was confirmed by GST pull-down assays and immunoprecipitation experiments. The Aurora-B protein level increased in HeLa cells cultured with proteasome inhibitor ALLN. Our data suggest that Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis.

Original language	English (US)
Pages (from-to)	157-162
Number of pages	6
Journal	Molecular and Cellular Biochemistry
Volume	254
Issue number	1-2
DOIs	https://doi.org/10.1023/A:1027317014159
State	Published - Dec 2003
Externally published	Yes

Keywords

Aurora-B
Degradation
HC8
Mitosis
Proteasome

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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10.1023/A:1027317014159

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title = "Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner",

abstract = "Human Aurora/Ip11-related kinase 2 (Aurora-B) is a key regulator of mitosis. Here human proteasome α-subunit C8 (HC8) was identified to interact with the Aurora-B by yeast two-hybrid screen. This finding was confirmed by GST pull-down assays and immunoprecipitation experiments. The Aurora-B protein level increased in HeLa cells cultured with proteasome inhibitor ALLN. Our data suggest that Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis.",

keywords = "Aurora-B, Degradation, HC8, Mitosis, Proteasome",

author = "Fengjue Shu and Shuguang Guo and Yongjun Dang and Meiyan Qi and Guangjin Zhou and Zekun Guo and Ying Zhang and Chaoqun Wu and Shouyuan Zhao and Long Yu",

note = "Funding Information: This study was supported by National 973 Program, 863 High Technology Program, and the National Natural Science Foundation of China. We are grateful to Dr. Yimin Hua, Dr. Jianqian Yu and Mr. Yi Wei for their invaluable technical assistance. We thank Dr. Youheng Wei for gene sequencing. We also give special thanks to Dr. Fangyan Dai, Dr. Hua He, Dr. Qing Ji, Dr. Yun Ding and Dr. Maoxian Deng for their helpful discussions.",

year = "2003",

month = dec,

doi = "10.1023/A:1027317014159",

language = "English (US)",

volume = "254",

pages = "157--162",

journal = "Molecular and Cellular Biochemistry",

issn = "0300-8177",

publisher = "Springer",

number = "1-2",

}

TY - JOUR

T1 - Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner

AU - Shu, Fengjue

AU - Guo, Shuguang

AU - Dang, Yongjun

AU - Qi, Meiyan

AU - Zhou, Guangjin

AU - Guo, Zekun

AU - Zhang, Ying

AU - Wu, Chaoqun

AU - Zhao, Shouyuan

AU - Yu, Long

N1 - Funding Information: This study was supported by National 973 Program, 863 High Technology Program, and the National Natural Science Foundation of China. We are grateful to Dr. Yimin Hua, Dr. Jianqian Yu and Mr. Yi Wei for their invaluable technical assistance. We thank Dr. Youheng Wei for gene sequencing. We also give special thanks to Dr. Fangyan Dai, Dr. Hua He, Dr. Qing Ji, Dr. Yun Ding and Dr. Maoxian Deng for their helpful discussions.

PY - 2003/12

Y1 - 2003/12

N2 - Human Aurora/Ip11-related kinase 2 (Aurora-B) is a key regulator of mitosis. Here human proteasome α-subunit C8 (HC8) was identified to interact with the Aurora-B by yeast two-hybrid screen. This finding was confirmed by GST pull-down assays and immunoprecipitation experiments. The Aurora-B protein level increased in HeLa cells cultured with proteasome inhibitor ALLN. Our data suggest that Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis.

AB - Human Aurora/Ip11-related kinase 2 (Aurora-B) is a key regulator of mitosis. Here human proteasome α-subunit C8 (HC8) was identified to interact with the Aurora-B by yeast two-hybrid screen. This finding was confirmed by GST pull-down assays and immunoprecipitation experiments. The Aurora-B protein level increased in HeLa cells cultured with proteasome inhibitor ALLN. Our data suggest that Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis.

KW - Aurora-B

KW - Degradation

KW - HC8

KW - Mitosis

KW - Proteasome

UR - https://www.scopus.com/pages/publications/0345328289

UR - https://www.scopus.com/pages/publications/0345328289#tab=citedBy

U2 - 10.1023/A:1027317014159

DO - 10.1023/A:1027317014159

M3 - Article

C2 - 14674694

AN - SCOPUS:0345328289

SN - 0300-8177

VL - 254

SP - 157

EP - 162

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

IS - 1-2

ER -

Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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