@article{d8ea89acdccf4ee7a9c2fb33ecaf9f59,
title = "HPV in situ hybridization with catalyzed signal amplification and polymerase chain reaction in establishing cerebellar metastasis of a cervical carcinoma",
abstract = "We report an unusual case of cerebellar metastasis from a cervical adenosquamous carcinoma in which molecular techniques assisted in establishing the correct diagnosis. The patient was a 43-year-old woman with surgically unresectable cervical carcinoma diagnosed 2 years before presenting with neurological symptoms. A magnetic resonance imaging scan showed a large, enhancing cerebellar lesion with significant brain stem compression. The excised cerebellar tumor resembled a small cell carcinoma and was initially not thought to be a metastasis from the cervical adenosquamous carcinoma. In situ hybridization with catalyzed signal amplification and polymerase chain reactions with primers specific for human papilloma virus (HPV) types 16 and 18 were used to determine the relationship between the cervical and the cerebellar neoplasms. A positive signal was present in the nuclei of both neoplasms by in situ hybridization using HPV16/18 DNA probes. Polymerase chain reaction revealed the presence of HPV- 18 DNA sequences in the cervical and cerebellar neoplasms confirming that the cerebellar neoplasm was a metastasis from the cervical primary.",
keywords = "Brain metastasis, Catalyzed signal amplification, Cervical carcinoma, Human papillomavirus, In situ hybridization, Polymerase chain reaction",
author = "Huang, {Chao Cheng} and Kashima, {Matthew L.} and Haiyan Chen and Shih, {I. E.Ming} and Kurman, {Robert J.} and Wu, {T. C.}",
note = "Funding Information: Cervical cancer is one of the most common cancers in women throughout the world. Despite advances in detection and management, cervical cancer affects 13,500 women and accounts for 4,500 deaths annually in the United States. Spread of cervical carcinoma usually occurs by direct local invasion into adjacent tissues and lymphatics and less commonly hematogenously. 1 Extranodal metastases usually involve liver, lung, and bone, in order of frequency. 2 Brain metastases are rare, with less than 70 reported cases in the literature. 24 Retrospective studies reveal the mean time from From the Departments of Pathology, Otolaryngology--Head and Neck Surgery, Obstetrics and Gynecology, Oncology, and Molecular Microbiology and Immunology, The Johns Hopkins Medical Institutions, Baltimore, MD and the Department of Pathology, Chang Gung Memorial Hospital, Kaohsiung, Taiwan. Supported by NIH 5 pol 34582-01 and the Richard W. TeLinde endowment from the Johns Hopkins University School of Medicine. Address correspondence and reprint requests to Dr. T.-C. Wu, Department of Pathology, The Johns Hopkins Hospital, Ross Bldg. Room 644, 720 Rutland Avenue, Baltimore, Maryland 21205. Copyright {\textcopyright} 1999 by W.B. Saunders Company 0046-8177/99/3005-0019510.00/0",
year = "1999",
doi = "10.1016/S0046-8177(99)90206-X",
language = "English (US)",
volume = "30",
pages = "587--591",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "5",
}