HPLC-MS/MS shotgun proteomic research of deer antlers with multiparallel protein extraction methods

Liang Gao, Dingyin Tao, Yichu Shan, Zhen Liang, Lihua Zhang, Yushu Huo, Yukui Zhang

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Deer antlers mature rapidly in 60 days, and subsequently shed in 5 days with rapid ossification. During this procedure, the function of deer antlers changes significantly. Therefore, the profiling of antler proteome is helpful to discover important growing and shedding regulation proteins, which might be of great significance for studying development and regeneration. In this study, a parallel protein extraction strategy was developed to extract proteins from antlers of red deer with five different lysis solutions, followed by shotgun proteomic analysis by microflow reversed-phase liquid chromatography/electrospray ionization/tandem mass spectrometry (μRPLC-ESI-MS/MS) with a 30. cm-long serially coupled microcolumn. Our experimental results showed that the identified proteins extracted by five kinds of lysis solution were complementary to each other. In total, 416 unique proteins were identified, with relative molecular masses from 2000 to 600,000, and isoelectric points from 3.84 to 11.57. All these results demonstrate that the combination of parallel protein extraction strategy and μRPLC-ESI-MS/MS analysis with serially coupled long microcolumns might be of great significance for comprehensive proteomic research of deer antler.

Original languageEnglish (US)
Pages (from-to)3370-3374
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Issue number32
StatePublished - Dec 15 2010
Externally publishedYes


  • Deer antler
  • Parallel protein extraction
  • Serially coupled microcolumn
  • Shotgun proteomic research

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology


Dive into the research topics of 'HPLC-MS/MS shotgun proteomic research of deer antlers with multiparallel protein extraction methods'. Together they form a unique fingerprint.

Cite this