Homology between mammalian cell DNA sequences and human herpesvirus genomes detected by a hybridization procedure with high-complexity probe

Keith Peden; Phoebe Mounts; Gary S. Hayward

doi:10.1016/0092-8674(82)90406-8

Homology between mammalian cell DNA sequences and human herpesvirus genomes detected by a hybridization procedure with high-complexity probe

Keith Peden, Phoebe Mounts, Gary S. Hayward

School of Medicine

Research output: Contribution to journal › Article › peer-review

119 Scopus citations

Abstract

Mouse and human DNA used as in vitro-labeled "high-complexity" probes revealed hybridization between specific herpesvirus DNA fragments on Southern transfers and repetitive sequences present at 10³ to 10⁵ copies per mammalian cell genome. Several different sites of major cell-virus sequence homology have been detected in both the herpes simplex virus type 1 and type 2 genomes, and these are located predominantly within the L and S inverted repeat regions and near the center of the L unique region. The hybrids persisted even in relatively stringent conditions, and appear to correlate closely with some of the previously recognized regions of size heterogeneity in the viral genome. Cloned viral DNA fragments from each site hybridized to different sets of discrete bands and dispersed elements within restriction-endonuclease-digested genomic DNA from a variety of vertebrate species. Localized cell-virus homology was also detected in both the human Epstein-Barr virus and cytomegalovirus genomes.

Original language	English (US)
Pages (from-to)	71-80
Number of pages	10
Journal	Cell
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(82)90406-8
State	Published - Nov 1982

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1016/0092-8674(82)90406-8

Cite this

@article{5a2a0e56d98742c08ea7cb5f05dd2428,

title = "Homology between mammalian cell DNA sequences and human herpesvirus genomes detected by a hybridization procedure with high-complexity probe",

abstract = "Mouse and human DNA used as in vitro-labeled {"}high-complexity{"} probes revealed hybridization between specific herpesvirus DNA fragments on Southern transfers and repetitive sequences present at 103 to 105 copies per mammalian cell genome. Several different sites of major cell-virus sequence homology have been detected in both the herpes simplex virus type 1 and type 2 genomes, and these are located predominantly within the L and S inverted repeat regions and near the center of the L unique region. The hybrids persisted even in relatively stringent conditions, and appear to correlate closely with some of the previously recognized regions of size heterogeneity in the viral genome. Cloned viral DNA fragments from each site hybridized to different sets of discrete bands and dispersed elements within restriction-endonuclease-digested genomic DNA from a variety of vertebrate species. Localized cell-virus homology was also detected in both the human Epstein-Barr virus and cytomegalovirus genomes.",

author = "Keith Peden and Phoebe Mounts and Hayward, {Gary S.}",

note = "Funding Information: We would like to thank Dan Nathans and Tom Kelly for their indulgence in allowing us to carry out experiments in their laboratories, and for reading the manuscript. We thank Dolores Ciufo for excellent technical assistance, Michael McLane for photographic work and Nancy Standish for typing the manuscript. Gifts of cell DNA preparations from Roy Schmickel (University of Michigan), Rob Saint (University of Adelaide). Chris Darnborough (University of Edinburgh) and Peter Ford (University of Edinburgh) are gratefully acknowledged. P. M. was supported by an NIH postdoctoral training grant; K. P. was supported initially by postdoctoral fellowships from the International Agency for Research on Cancer and the Science Research Council of Great Britain. This work was supported by grants from the National Institutes of Health (to G. S. H. and D. Nathans).",

year = "1982",

month = nov,

doi = "10.1016/0092-8674(82)90406-8",

language = "English (US)",

volume = "31",

pages = "71--80",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Homology between mammalian cell DNA sequences and human herpesvirus genomes detected by a hybridization procedure with high-complexity probe

AU - Peden, Keith

AU - Mounts, Phoebe

AU - Hayward, Gary S.

N1 - Funding Information: We would like to thank Dan Nathans and Tom Kelly for their indulgence in allowing us to carry out experiments in their laboratories, and for reading the manuscript. We thank Dolores Ciufo for excellent technical assistance, Michael McLane for photographic work and Nancy Standish for typing the manuscript. Gifts of cell DNA preparations from Roy Schmickel (University of Michigan), Rob Saint (University of Adelaide). Chris Darnborough (University of Edinburgh) and Peter Ford (University of Edinburgh) are gratefully acknowledged. P. M. was supported by an NIH postdoctoral training grant; K. P. was supported initially by postdoctoral fellowships from the International Agency for Research on Cancer and the Science Research Council of Great Britain. This work was supported by grants from the National Institutes of Health (to G. S. H. and D. Nathans).

PY - 1982/11

Y1 - 1982/11

N2 - Mouse and human DNA used as in vitro-labeled "high-complexity" probes revealed hybridization between specific herpesvirus DNA fragments on Southern transfers and repetitive sequences present at 103 to 105 copies per mammalian cell genome. Several different sites of major cell-virus sequence homology have been detected in both the herpes simplex virus type 1 and type 2 genomes, and these are located predominantly within the L and S inverted repeat regions and near the center of the L unique region. The hybrids persisted even in relatively stringent conditions, and appear to correlate closely with some of the previously recognized regions of size heterogeneity in the viral genome. Cloned viral DNA fragments from each site hybridized to different sets of discrete bands and dispersed elements within restriction-endonuclease-digested genomic DNA from a variety of vertebrate species. Localized cell-virus homology was also detected in both the human Epstein-Barr virus and cytomegalovirus genomes.

AB - Mouse and human DNA used as in vitro-labeled "high-complexity" probes revealed hybridization between specific herpesvirus DNA fragments on Southern transfers and repetitive sequences present at 103 to 105 copies per mammalian cell genome. Several different sites of major cell-virus sequence homology have been detected in both the herpes simplex virus type 1 and type 2 genomes, and these are located predominantly within the L and S inverted repeat regions and near the center of the L unique region. The hybrids persisted even in relatively stringent conditions, and appear to correlate closely with some of the previously recognized regions of size heterogeneity in the viral genome. Cloned viral DNA fragments from each site hybridized to different sets of discrete bands and dispersed elements within restriction-endonuclease-digested genomic DNA from a variety of vertebrate species. Localized cell-virus homology was also detected in both the human Epstein-Barr virus and cytomegalovirus genomes.

UR - http://www.scopus.com/inward/record.url?scp=0020407616&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020407616&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(82)90406-8

DO - 10.1016/0092-8674(82)90406-8

M3 - Article

C2 - 6297753

AN - SCOPUS:0020407616

SN - 0092-8674

VL - 31

SP - 71

EP - 80

JO - Cell

JF - Cell

IS - 1

ER -

Homology between mammalian cell DNA sequences and human herpesvirus genomes detected by a hybridization procedure with high-complexity probe

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this