Homing and clonogenic outgrowth of CD34+ peripheral blood stem cells: A role for L-selectin?

Fransien De Boer, Floortje L. Kessler, Tanja Netelenbos, Sonja Zweegman, Peter C. Huijgens, Elsken Van der Wall, Jan A.M. Van der Linden, Herbert M. Pinedo, Gerrit Jan Schuurhuis, Angelika M. Dräger

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Objective. After transplantation of hematopoietic stem cells, adhesion molecules play a major role in the multistep process of engraftment in which L-selectin is suggested to be of relevance. A positive correlation previously was found between the number of reinfused L-selectin+ stem cells and platelet recovery. In the present study, we determined the role of L-selectin in different engraftment steps, i.e., adhesion to endothelial cells, migration, and clonogenic outgrowth by in vitro assays that closely mimic the in vivo situation. Materials and Methods. Flow adhesion and migration experiments were performed using the human bone marrow endothelial cell line 4LHBMEC and isolated peripheral CD34+ cells with or without blocking of L-selectin-ligand interaction. Various clonogenic assays, including serum-free colony-forming unit-megakaryocytes (CFU-MK) and burst-forming unit-megakaryocytes (BFU-MK), were performed with sorted L-selectin+L-selectin- cells or in the presence of antibodies. Results. Blocking of L-selectin on CD34+ cells did not significantly affect rolling over and firm adhesion to 4LHBMEC. In addition, no role for L-selectin was found in transendothelial migration experiments. Finally, in clonogenic outgrowth of sorted or anti-L-selectin monoclonal antibody-incubated CD34+ cells, no key role for L-selectin expression could be defined in BFU-MK and CFU-MK assays. Conclusion. Using in vitro assays for CD34+ stem cell adhesion, migration, and clonogenic capacity, we were not able to define a major role for L-selectin.

Original languageEnglish (US)
Pages (from-to)590-597
Number of pages8
JournalExperimental Hematology
Volume30
Issue number6
DOIs
StatePublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

Fingerprint

Dive into the research topics of 'Homing and clonogenic outgrowth of CD34+ peripheral blood stem cells: A role for L-selectin?'. Together they form a unique fingerprint.

Cite this