Homer2 and Homer3 Act as Novel Biomarkers in Diagnosis of hepatitis B virus-induced Hepatocellular Carcinoma

Ping Luo; Chunzi Liang; Wei Jing; Man Zhu; Hu Zhou; Hongyan Chai; Paul F. Worley; Jiancheng Tu

doi:10.7150/JCA.52118

Homer2 and Homer3 Act as Novel Biomarkers in Diagnosis of hepatitis B virus-induced Hepatocellular Carcinoma

Ping Luo, Chunzi Liang, Wei Jing, Man Zhu, Hu Zhou, Hongyan Chai, Paul F. Worley, Jiancheng Tu

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-related mortality worldwide. Early detection of HCC can significantly improve patients' outcomes. An increasing number of studies have validated that Homer is dysregulated in cancers and may serve as diagnostic markers. In the present study, we investigated the expression profile and diagnostic significance of Homer2 and Homer3 in hepatitis B virus-induced HCC (HBV-HCC). Methods: Quantitative real-time PCR (QRT-PCR), western blot analysis and immunohistochemistry analysis. Results: Homer2 and Homer3 were downregulated in HCC. The expression of Homer2 was associated with tumor differentiation grade (P= 0.012) and total protein (TP) level (P= 0.032). Homer3 was related to tumor size (P= 0.010), tumor nodes (P= 0.026) and γ-glutamyl transferase (GGT) level (P= 0.001). The receiver operating characteristic curve analyses indicated that the combination of Homer2, Homer3 and AFP possessed a high accuracy (AUC=0.900) to diagnose HCC cases from healthy controls. Conclusion: Our data indicated that Homer2 and Homer3 were downregulated in HCC and might be potential diagnostic marker for HCC.

Original language	English (US)
Pages (from-to)	3439-3447
Number of pages	9
Journal	Journal of Cancer
Volume	12
Issue number	12
DOIs	https://doi.org/10.7150/JCA.52118
State	Published - 2021

Keywords

Diagnosis
HCC
Homer2
Homer3
Tumorigenesis

ASJC Scopus subject areas

Oncology

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@article{8ff2ca00dbc74ec7ad475b8074002f38,

title = "Homer2 and Homer3 Act as Novel Biomarkers in Diagnosis of hepatitis B virus-induced Hepatocellular Carcinoma",

abstract = "Background: Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-related mortality worldwide. Early detection of HCC can significantly improve patients' outcomes. An increasing number of studies have validated that Homer is dysregulated in cancers and may serve as diagnostic markers. In the present study, we investigated the expression profile and diagnostic significance of Homer2 and Homer3 in hepatitis B virus-induced HCC (HBV-HCC). Methods: Quantitative real-time PCR (QRT-PCR), western blot analysis and immunohistochemistry analysis. Results: Homer2 and Homer3 were downregulated in HCC. The expression of Homer2 was associated with tumor differentiation grade (P= 0.012) and total protein (TP) level (P= 0.032). Homer3 was related to tumor size (P= 0.010), tumor nodes (P= 0.026) and γ-glutamyl transferase (GGT) level (P= 0.001). The receiver operating characteristic curve analyses indicated that the combination of Homer2, Homer3 and AFP possessed a high accuracy (AUC=0.900) to diagnose HCC cases from healthy controls. Conclusion: Our data indicated that Homer2 and Homer3 were downregulated in HCC and might be potential diagnostic marker for HCC.",

keywords = "Diagnosis, HCC, Homer2, Homer3, Tumorigenesis",

author = "Ping Luo and Chunzi Liang and Wei Jing and Man Zhu and Hu Zhou and Hongyan Chai and Worley, {Paul F.} and Jiancheng Tu",

note = "Funding Information: This study was supported by the Research of Homer ELISA Kit for Breast Cancer (201260523173-2), Science and Technology Innovation Cultivation Fund of Zhongnan Hospital of Wuhan University (znpy2018067) and National Basic Research Program of China (973 Program) (2012CB720605). Publisher Copyright: {\textcopyright} 2021 Ivyspring International Publisher. All rights reserved.",

year = "2021",

doi = "10.7150/JCA.52118",

language = "English (US)",

volume = "12",

pages = "3439--3447",

journal = "Journal of Cancer",

issn = "1837-9664",

publisher = "Ivyspring International Publisher",

number = "12",

}

TY - JOUR

T1 - Homer2 and Homer3 Act as Novel Biomarkers in Diagnosis of hepatitis B virus-induced Hepatocellular Carcinoma

AU - Luo, Ping

AU - Liang, Chunzi

AU - Jing, Wei

AU - Zhu, Man

AU - Zhou, Hu

AU - Chai, Hongyan

AU - Worley, Paul F.

AU - Tu, Jiancheng

N1 - Funding Information: This study was supported by the Research of Homer ELISA Kit for Breast Cancer (201260523173-2), Science and Technology Innovation Cultivation Fund of Zhongnan Hospital of Wuhan University (znpy2018067) and National Basic Research Program of China (973 Program) (2012CB720605). Publisher Copyright: © 2021 Ivyspring International Publisher. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Background: Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-related mortality worldwide. Early detection of HCC can significantly improve patients' outcomes. An increasing number of studies have validated that Homer is dysregulated in cancers and may serve as diagnostic markers. In the present study, we investigated the expression profile and diagnostic significance of Homer2 and Homer3 in hepatitis B virus-induced HCC (HBV-HCC). Methods: Quantitative real-time PCR (QRT-PCR), western blot analysis and immunohistochemistry analysis. Results: Homer2 and Homer3 were downregulated in HCC. The expression of Homer2 was associated with tumor differentiation grade (P= 0.012) and total protein (TP) level (P= 0.032). Homer3 was related to tumor size (P= 0.010), tumor nodes (P= 0.026) and γ-glutamyl transferase (GGT) level (P= 0.001). The receiver operating characteristic curve analyses indicated that the combination of Homer2, Homer3 and AFP possessed a high accuracy (AUC=0.900) to diagnose HCC cases from healthy controls. Conclusion: Our data indicated that Homer2 and Homer3 were downregulated in HCC and might be potential diagnostic marker for HCC.

AB - Background: Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-related mortality worldwide. Early detection of HCC can significantly improve patients' outcomes. An increasing number of studies have validated that Homer is dysregulated in cancers and may serve as diagnostic markers. In the present study, we investigated the expression profile and diagnostic significance of Homer2 and Homer3 in hepatitis B virus-induced HCC (HBV-HCC). Methods: Quantitative real-time PCR (QRT-PCR), western blot analysis and immunohistochemistry analysis. Results: Homer2 and Homer3 were downregulated in HCC. The expression of Homer2 was associated with tumor differentiation grade (P= 0.012) and total protein (TP) level (P= 0.032). Homer3 was related to tumor size (P= 0.010), tumor nodes (P= 0.026) and γ-glutamyl transferase (GGT) level (P= 0.001). The receiver operating characteristic curve analyses indicated that the combination of Homer2, Homer3 and AFP possessed a high accuracy (AUC=0.900) to diagnose HCC cases from healthy controls. Conclusion: Our data indicated that Homer2 and Homer3 were downregulated in HCC and might be potential diagnostic marker for HCC.

KW - Diagnosis

KW - HCC

KW - Homer2

KW - Homer3

KW - Tumorigenesis

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DO - 10.7150/JCA.52118

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SN - 1837-9664

VL - 12

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EP - 3447

JO - Journal of Cancer

JF - Journal of Cancer

IS - 12

ER -

Homer2 and Homer3 Act as Novel Biomarkers in Diagnosis of hepatitis B virus-induced Hepatocellular Carcinoma

Abstract

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