HIV-1 Tat neurotoxicity in primary cultures of rat midbrain fetal neurons: Changes in dopamine transporter binding and immunoreactivity

Marina V. Aksenova, Janelle M. Silvers, Michael Y. Aksenov, Avindra Nath, Philip D. Ray, Charles F. Mactutus, Rosemarie M. Booze

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

HIV-1 neurotoxic proteins (Tat, gp120) are believed to play a major role in pathogenesis of dementia in a significant portion of the AIDS patient population. Dopaminergic systems appear to be particularly important in HIV-associated dementia. In the current studies, we determined that primary cell cultures prepared from the midbrain of 18-day-old rat fetuses are sensitive to Tat neurotoxicity and investigated the possible effects of Tat on DAT-specific ligand binding and DAT immunoreactivity in rat fetal midbrain cultures. We found that Tat neurotoxicity was associated with a significant decrease in [3H]WIN 35428 binding. Immunostaining of cell cultures with antibodies recognizing the C-end epitope of DAT did not reveal significant changes in DAT immunoreactivity. The results of this study implicate involvement of monoamine transmission systems in HIV-associated dementia.

Original languageEnglish (US)
Pages (from-to)235-239
Number of pages5
JournalNeuroscience Letters
Volume395
Issue number3
DOIs
StatePublished - Mar 13 2006
Externally publishedYes

Keywords

  • DAT
  • Monoamines
  • Radioligand binding
  • Viral proteins

ASJC Scopus subject areas

  • General Neuroscience

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