HIV-1 DNA is detected in bone marrow populations containing CD4 + T cells but is not found in purified CD34 + hematopoietic progenitor cells in most patients on antiretroviral therapy

Christine M. Durand; Gabriel Ghiaur; Janet D. Siliciano; S. Alireza Rabi; Evelyn E. Eisele; Maria Salgado; Liang Shan; Jun F. Lai; Hao Zhang; Joseph Margolick; Richard J. Jones; Joel E. Gallant; Richard F. Ambinder; Robert F. Siliciano

doi:10.1093/infdis/jir884

HIV-1 DNA is detected in bone marrow populations containing CD4 ⁺ T cells but is not found in purified CD34 ⁺ hematopoietic progenitor cells in most patients on antiretroviral therapy

Christine M. Durand, Gabriel Ghiaur, Janet D. Siliciano, S. Alireza Rabi, Evelyn E. Eisele, Maria Salgado, Liang Shan, Jun F. Lai, Hao Zhang, Joseph Margolick, Richard J. Jones, Joel E. Gallant, Richard F. Ambinder, Robert F. Siliciano

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Abstract

Identifying cellular reservoirs of human immunodeficiency virus type 1 (HIV-1) in patients on antiretroviral therapy (ART) is critical to finding a cure for HIV-1. In addition to resting CD4 ⁺ T cells, CD34 ⁺ hematopoietic progenitor cells have been proposed as another reservoir. We obtained bone marrow aspirates from 11 patients on ART who had undetectable plasma HIV-1 RNA. HIV-1 DNA was detected in CD4 ⁺ T cells from peripheral blood in all patients and from bone marrow cellular fractions containing T cells in most patients. We did not find HIV-1 DNA in highly purified CD34 ⁺ populations using either a sensitive real-time polymerase chain reaction assay or a coculture assay for replication-competent HIV-1.

Original language	English (US)
Pages (from-to)	1014-1018
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	205
Issue number	6
DOIs	https://doi.org/10.1093/infdis/jir884
State	Published - Mar 15 2012

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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10.1093/infdis/jir884

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Durand, C. M., Ghiaur, G., Siliciano, J. D., Rabi, S. A., Eisele, E. E., Salgado, M., Shan, L., Lai, J. F., Zhang, H., Margolick, J., Jones, R. J., Gallant, J. E., Ambinder, R. F., & Siliciano, R. F. (2012). HIV-1 DNA is detected in bone marrow populations containing CD4 ⁺ T cells but is not found in purified CD34 ⁺ hematopoietic progenitor cells in most patients on antiretroviral therapy. Journal of Infectious Diseases, 205(6), 1014-1018. https://doi.org/10.1093/infdis/jir884

HIV-1 DNA is detected in bone marrow populations containing CD4 ⁺ T cells but is not found in purified CD34 ⁺ hematopoietic progenitor cells in most patients on antiretroviral therapy. / Durand, Christine M.; Ghiaur, Gabriel ; Siliciano, Janet D. et al.
In: Journal of Infectious Diseases, Vol. 205, No. 6, 15.03.2012, p. 1014-1018.

Research output: Contribution to journal › Article › peer-review

Durand, CM , Ghiaur, G , Siliciano, JD, Rabi, SA, Eisele, EE, Salgado, M, Shan, L, Lai, JF, Zhang, H , Margolick, J , Jones, RJ, Gallant, JE, Ambinder, RF & Siliciano, RF 2012, 'HIV-1 DNA is detected in bone marrow populations containing CD4 ⁺ T cells but is not found in purified CD34 ⁺ hematopoietic progenitor cells in most patients on antiretroviral therapy', Journal of Infectious Diseases, vol. 205, no. 6, pp. 1014-1018. https://doi.org/10.1093/infdis/jir884

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title = "HIV-1 DNA is detected in bone marrow populations containing CD4 + T cells but is not found in purified CD34 + hematopoietic progenitor cells in most patients on antiretroviral therapy",

abstract = "Identifying cellular reservoirs of human immunodeficiency virus type 1 (HIV-1) in patients on antiretroviral therapy (ART) is critical to finding a cure for HIV-1. In addition to resting CD4 + T cells, CD34 + hematopoietic progenitor cells have been proposed as another reservoir. We obtained bone marrow aspirates from 11 patients on ART who had undetectable plasma HIV-1 RNA. HIV-1 DNA was detected in CD4 + T cells from peripheral blood in all patients and from bone marrow cellular fractions containing T cells in most patients. We did not find HIV-1 DNA in highly purified CD34 + populations using either a sensitive real-time polymerase chain reaction assay or a coculture assay for replication-competent HIV-1.",

author = "Durand, {Christine M.} and Gabriel Ghiaur and Siliciano, {Janet D.} and Rabi, {S. Alireza} and Eisele, {Evelyn E.} and Maria Salgado and Liang Shan and Lai, {Jun F.} and Hao Zhang and Joseph Margolick and Jones, {Richard J.} and Gallant, {Joel E.} and Ambinder, {Richard F.} and Siliciano, {Robert F.}",

note = "Funding Information: Financial support. This work was supported by the National Institutes of Health (NIH), National Cancer Institute (grants P30CA06973 and P01CA1539627); Howard Hughes Medical Institute and NIH; and National Institute of Allergy and Infectious Diseases (grant AI43222 to R. F. S). Potential conflicts of interest. All authors: No reported conflicts.",

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doi = "10.1093/infdis/jir884",

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AU - Ghiaur, Gabriel

AU - Siliciano, Janet D.

AU - Rabi, S. Alireza

AU - Eisele, Evelyn E.

AU - Salgado, Maria

AU - Shan, Liang

AU - Lai, Jun F.

AU - Zhang, Hao

AU - Margolick, Joseph

AU - Jones, Richard J.

AU - Gallant, Joel E.

AU - Ambinder, Richard F.

AU - Siliciano, Robert F.

N1 - Funding Information: Financial support. This work was supported by the National Institutes of Health (NIH), National Cancer Institute (grants P30CA06973 and P01CA1539627); Howard Hughes Medical Institute and NIH; and National Institute of Allergy and Infectious Diseases (grant AI43222 to R. F. S). Potential conflicts of interest. All authors: No reported conflicts.

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N2 - Identifying cellular reservoirs of human immunodeficiency virus type 1 (HIV-1) in patients on antiretroviral therapy (ART) is critical to finding a cure for HIV-1. In addition to resting CD4 + T cells, CD34 + hematopoietic progenitor cells have been proposed as another reservoir. We obtained bone marrow aspirates from 11 patients on ART who had undetectable plasma HIV-1 RNA. HIV-1 DNA was detected in CD4 + T cells from peripheral blood in all patients and from bone marrow cellular fractions containing T cells in most patients. We did not find HIV-1 DNA in highly purified CD34 + populations using either a sensitive real-time polymerase chain reaction assay or a coculture assay for replication-competent HIV-1.

AB - Identifying cellular reservoirs of human immunodeficiency virus type 1 (HIV-1) in patients on antiretroviral therapy (ART) is critical to finding a cure for HIV-1. In addition to resting CD4 + T cells, CD34 + hematopoietic progenitor cells have been proposed as another reservoir. We obtained bone marrow aspirates from 11 patients on ART who had undetectable plasma HIV-1 RNA. HIV-1 DNA was detected in CD4 + T cells from peripheral blood in all patients and from bone marrow cellular fractions containing T cells in most patients. We did not find HIV-1 DNA in highly purified CD34 + populations using either a sensitive real-time polymerase chain reaction assay or a coculture assay for replication-competent HIV-1.

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HIV-1 DNA is detected in bone marrow populations containing CD4 ⁺ T cells but is not found in purified CD34 ⁺ hematopoietic progenitor cells in most patients on antiretroviral therapy

Abstract

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