@inproceedings{230510414c9140418b4ed302a418b993,
title = "High-throughput single-cell pathogen detection on a droplet microfluidic platform",
abstract = "Digital microfluidics is rapidly becoming the microfluidic technology of choice for a large variety of applications [1]. The idea of using discrete droplets to maintain a high concentration of sub-cellular contents for digital detection at single molecule or cell level is highly promising as it can greatly enhance the detection sensitivity and quantification accuracy. However, realization of this idea has been hampered by the need for complicated target amplification and off-chip sample processing [2,3]. Here we extend the repertoire of digital microfluidic applications to include a versatile {"}sample-to-answer{"} platform for pathogen detection. The proposed platform can perform integrated sample preparation and amplification-free pathogen detection on a microfluidic chip. The integration enables digital detection in a continuous flow format and thereby high-throughput measurement of single cells.",
author = "Rane, {Tushar D.} and Helena Zec and Chris Puleo and Lee, {Abraham P.} and Wang, {Tza Huei}",
year = "2011",
doi = "10.1109/MEMSYS.2011.5734566",
language = "English (US)",
isbn = "9781424496327",
series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",
pages = "881--884",
booktitle = "2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011",
note = "24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 ; Conference date: 23-01-2011 Through 27-01-2011",
}