@inproceedings{516f549ee4214253aa2ba3feffed54c9,
title = "High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array",
abstract = "We performed on-chip DNA methylation analysis using methylation-specific PCR (MSP) within a high throughput microfluidic droplet array. The device uses of the oil phase as a companion fluid for both sample actuation and compartmentalization. These technical advantages allow for infusion of minute amounts of sample for arrayed MSP analysis, without the added complexities inherent in microfluidic droplet-based studies. Ease of use of this micro device is exemplified by analysis of two tumor suppressor promoters, p15 and TMS1 using an on-chip methylation assay. These results were consistent with standard MSP protocols, yet the simplicity of the droplet-in-oil microfluidic PCR platform provides an easy and efficient tool for DNA methylation analysis in a large-scale arrayed manner.",
keywords = "DNA methylation, Droplet-in-oil, PCR",
author = "Yi Zhang and Vasudev Bailey and Puleo, {Christopher M.} and Hariharan Easwaran and Elizabeth Griffiths and Herman, {James G.} and Baylin, {Stephen B.} and Wang, {Tza Huei}",
year = "2009",
doi = "10.1109/SENSOR.2009.5285699",
language = "English (US)",
isbn = "9781424441938",
series = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems",
pages = "806--808",
booktitle = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems",
note = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems ; Conference date: 21-06-2009 Through 25-06-2009",
}