High-resolution liquid chromatographic analysis of methylated purine and pyrimidine bases in transfer RNA

Duane B. Lakings; T. Phillip Waalkes; John E. Mrochek

doi:10.1016/S0021-9673(00)83702-4

High-resolution liquid chromatographic analysis of methylated purine and pyrimidine bases in transfer RNA

Duane B. Lakings, T. Phillip Waalkes, John E. Mrochek

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Methylated and major purine and pyridine bases were separated and quantified by high-resolution liquid chromatography after hydrolyzing transfer ribonucleic acids (tRNAs). Separation was accomplished by eluting the h ydrolyzed samples from an anion-exchange column with a concentration gradient of ammonium acetate at pH 9.2. Isolated samples of tRNA were hydrolyzed to the free bases with a triluoroacetic acid-formic acid mixture at 200°. Detection limits of 100-200 ng/ml were measured for the methylated bases; analytical data are reported for ten methylated bases plus the four major bases of calf liver and rat liver tRNA.

Original language	English (US)
Pages (from-to)	83-92
Number of pages	10
Journal	Journal of Chromatography A
Volume	116
Issue number	1
DOIs	https://doi.org/10.1016/S0021-9673(00)83702-4
State	Published - Jan 7 1976
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(00)83702-4

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abstract = "Methylated and major purine and pyridine bases were separated and quantified by high-resolution liquid chromatography after hydrolyzing transfer ribonucleic acids (tRNAs). Separation was accomplished by eluting the h ydrolyzed samples from an anion-exchange column with a concentration gradient of ammonium acetate at pH 9.2. Isolated samples of tRNA were hydrolyzed to the free bases with a triluoroacetic acid-formic acid mixture at 200°. Detection limits of 100-200 ng/ml were measured for the methylated bases; analytical data are reported for ten methylated bases plus the four major bases of calf liver and rat liver tRNA.",

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N2 - Methylated and major purine and pyridine bases were separated and quantified by high-resolution liquid chromatography after hydrolyzing transfer ribonucleic acids (tRNAs). Separation was accomplished by eluting the h ydrolyzed samples from an anion-exchange column with a concentration gradient of ammonium acetate at pH 9.2. Isolated samples of tRNA were hydrolyzed to the free bases with a triluoroacetic acid-formic acid mixture at 200°. Detection limits of 100-200 ng/ml were measured for the methylated bases; analytical data are reported for ten methylated bases plus the four major bases of calf liver and rat liver tRNA.

AB - Methylated and major purine and pyridine bases were separated and quantified by high-resolution liquid chromatography after hydrolyzing transfer ribonucleic acids (tRNAs). Separation was accomplished by eluting the h ydrolyzed samples from an anion-exchange column with a concentration gradient of ammonium acetate at pH 9.2. Isolated samples of tRNA were hydrolyzed to the free bases with a triluoroacetic acid-formic acid mixture at 200°. Detection limits of 100-200 ng/ml were measured for the methylated bases; analytical data are reported for ten methylated bases plus the four major bases of calf liver and rat liver tRNA.

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High-resolution liquid chromatographic analysis of methylated purine and pyrimidine bases in transfer RNA

Abstract

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