TY - JOUR
T1 - High rates of placental inflammation among samples collected by the Multi-Omics for Mothers and Infants consortium
AU - Robinson, Joshua F.
AU - Das, Sayan
AU - Khan, Waqasuddin
AU - Khanam, Rasheda
AU - Price, Joan T.
AU - Rahman, Anisur
AU - Ahmed, Salahuddin
AU - Ali, Said Mohammed
AU - Deb, Saikat
AU - Deveale, Brian
AU - Dutta, Arup
AU - Gormley, Matthew
AU - Hall, Steven C.
AU - Hasan, A. S.M.Tarik
AU - Hotwani, Aneeta
AU - Juma, Mohamed Hamid
AU - Kasaro, Margaret P.
AU - Khalid, Javairia
AU - Kshetrapal, Pallavi
AU - McMaster, Michael T.
AU - Mehmood, Usma
AU - Nisar, Imran
AU - Pervin, Jesmin
AU - Rahman, Sayedur
AU - Raqib, Rubhana
AU - San, Ali
AU - Sarker, Protim
AU - Tuomivaara, Sami T.
AU - Zhang, Ge
AU - Zhou, Yan
AU - Aktar, Shaki
AU - Baqui, Abdullah H.
AU - Jehan, Fyezah
AU - Sazawal, Sunil
AU - Stringer, Jeffrey S.A.
AU - Fisher, Susan J.
N1 - Publisher Copyright:
© 2024 The Author(s)
PY - 2024
Y1 - 2024
N2 - Background: The Multi-Omics for Mothers and Infants consortium aims to improve birth outcomes. Preterm birth is a major obstetrical complication globally and causes significant infant and childhood morbidity and mortality. Objective: We analyzed placental samples (basal plate, placenta or chorionic villi, and the chorionic plate) collected by the 5 Multi-Omics for Mothers and Infants sites, namely The Alliance for Maternal and Newborn Health Improvement Bangladesh, The Alliance for Maternal and Newborn Health Improvement Pakistan, The Alliance for Maternal and Newborn Health Improvement Tanzania, The Global Alliance to Prevent Prematurity and Stillbirth Bangladesh, and The Global Alliance to Prevent Prematurity and Stillbirth Zambia. The goal was to analyze the morphology and gene expression of samples collected from preterm and uncomplicated term births. Study Design: The teams provided biopsies from 166 singleton preterm (<37 weeks’ gestation) and 175 term (≥37 weeks’ gestation) deliveries. The samples were fixed in formalin and paraffin embedded. Tissue sections from these samples were stained with hematoxylin and eosin and subjected to morphologic analyses. Other placental biopsies (n=35 preterm, 21 term) were flash frozen, which enabled RNA purification for bulk transcriptomics. Results: The morphologic analyses revealed a surprisingly high rate of inflammation that involved the basal plate, placenta or chorionic villi, and the chorionic plate. The rate of inflammation in chorionic villus samples, likely attributable to chronic villitis, ranged from 25% (Pakistan site) to 60% (Zambia site) of cases. Leukocyte infiltration in this location vs in the basal plate or chorionic plate correlated with preterm birth. Our transcriptomic analyses identified 267 genes that were differentially expressed between placentas from preterm vs those from term births (123 upregulated, 144 downregulated). Mapping the differentially expressed genes onto single-cell RNA sequencing data from human placentas suggested that all the component cell types, either singly or in subsets, contributed to the observed dysregulation. Consistent with the histopathologic findings, gene ontology analyses highlighted the presence of leukocyte infiltration or activation and inflammatory responses in both the fetal and maternal compartments. Conclusion: The relationship between placental inflammation and preterm birth is appreciated in developed countries. In this study, we showed that this link also exists in developing geographies. In addition, among the participating sites, we found geographic- and population-based differences in placental inflammation and preterm birth, suggesting the importance of local factors.
AB - Background: The Multi-Omics for Mothers and Infants consortium aims to improve birth outcomes. Preterm birth is a major obstetrical complication globally and causes significant infant and childhood morbidity and mortality. Objective: We analyzed placental samples (basal plate, placenta or chorionic villi, and the chorionic plate) collected by the 5 Multi-Omics for Mothers and Infants sites, namely The Alliance for Maternal and Newborn Health Improvement Bangladesh, The Alliance for Maternal and Newborn Health Improvement Pakistan, The Alliance for Maternal and Newborn Health Improvement Tanzania, The Global Alliance to Prevent Prematurity and Stillbirth Bangladesh, and The Global Alliance to Prevent Prematurity and Stillbirth Zambia. The goal was to analyze the morphology and gene expression of samples collected from preterm and uncomplicated term births. Study Design: The teams provided biopsies from 166 singleton preterm (<37 weeks’ gestation) and 175 term (≥37 weeks’ gestation) deliveries. The samples were fixed in formalin and paraffin embedded. Tissue sections from these samples were stained with hematoxylin and eosin and subjected to morphologic analyses. Other placental biopsies (n=35 preterm, 21 term) were flash frozen, which enabled RNA purification for bulk transcriptomics. Results: The morphologic analyses revealed a surprisingly high rate of inflammation that involved the basal plate, placenta or chorionic villi, and the chorionic plate. The rate of inflammation in chorionic villus samples, likely attributable to chronic villitis, ranged from 25% (Pakistan site) to 60% (Zambia site) of cases. Leukocyte infiltration in this location vs in the basal plate or chorionic plate correlated with preterm birth. Our transcriptomic analyses identified 267 genes that were differentially expressed between placentas from preterm vs those from term births (123 upregulated, 144 downregulated). Mapping the differentially expressed genes onto single-cell RNA sequencing data from human placentas suggested that all the component cell types, either singly or in subsets, contributed to the observed dysregulation. Consistent with the histopathologic findings, gene ontology analyses highlighted the presence of leukocyte infiltration or activation and inflammatory responses in both the fetal and maternal compartments. Conclusion: The relationship between placental inflammation and preterm birth is appreciated in developed countries. In this study, we showed that this link also exists in developing geographies. In addition, among the participating sites, we found geographic- and population-based differences in placental inflammation and preterm birth, suggesting the importance of local factors.
KW - chorionic villi
KW - chronic villitis
KW - inflammation
KW - parturition
KW - placenta
KW - pregnancy
KW - prematurity preterm birth
KW - transcriptomics
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UR - http://www.scopus.com/inward/citedby.url?scp=85194046655&partnerID=8YFLogxK
U2 - 10.1016/j.ajog.2024.04.034
DO - 10.1016/j.ajog.2024.04.034
M3 - Article
C2 - 38697337
AN - SCOPUS:85194046655
SN - 0002-9378
JO - American journal of obstetrics and gynecology
JF - American journal of obstetrics and gynecology
ER -