High precision profiling of glycopeptides using HPLC-CHIP/MS TOF

Kevin Killeen, Hongfeng Yin, Reid Brennen, Karen Seaward, Rudi Grimm, Xiao Jun Li, Hui Zhang, Ruedi Aebersold

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Protein glycosylation is critically important to biological function and disease state. An effective method for the selective isolation of N-linked glycopeptides has been developed, based on the conjugation of glycoproteins to a solid support using hydrazine chemistry [1]. Due to extreme sample complexity, separation methods such as nano-LC/MS has been used as an analytical tool for human serum samples but we illustrate here that a microfluidic device can achieve superior performance in both sensitivity and reproducibility for profiling complex samples such as glycosylated proteins.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages274-276
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Externally publishedYes
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period10/9/0510/13/05

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Fingerprint

Dive into the research topics of 'High precision profiling of glycopeptides using HPLC-CHIP/MS TOF'. Together they form a unique fingerprint.

Cite this