TY - JOUR
T1 - High and low affinity binding sites for endothelin on cultured rat glomerular mesangial cells
AU - Badr, Kamal F.
AU - Munger, Karen A.
AU - Sugiura, Masanori
AU - Snajdar, Rudolf M.
AU - Schwartzberg, Mindy
AU - Inagami, Tadashi
PY - 1989/6/15
Y1 - 1989/6/15
N2 - Endothelin contracts glomerular mesangial cells, thereby influencing glomerular size and filtration rate. Here, we demonstrate the presence of two ET-specific binding sites on cultured rat mesangial cells with Kds of 0.76 and 44.70 nM, and maximal binding capacity (Bmax) values of 6.78×102 and 27.60×102 binding sites/cell, respectively. Binding of [125I]-ET was maximal at 120 min at 4°C, stable for the subsequent 60 min, and selective. No competition for binding was observed with >1000-fold concentrations of atrial natriuretic peptide, angiotensin II, arginine vasopressin, nicardipine, or nifedipine. The presence of specific receptors for ET on glomerular mesangial cells suggests a major role for this peptide in the regulation of glomerular filtration rate.
AB - Endothelin contracts glomerular mesangial cells, thereby influencing glomerular size and filtration rate. Here, we demonstrate the presence of two ET-specific binding sites on cultured rat mesangial cells with Kds of 0.76 and 44.70 nM, and maximal binding capacity (Bmax) values of 6.78×102 and 27.60×102 binding sites/cell, respectively. Binding of [125I]-ET was maximal at 120 min at 4°C, stable for the subsequent 60 min, and selective. No competition for binding was observed with >1000-fold concentrations of atrial natriuretic peptide, angiotensin II, arginine vasopressin, nicardipine, or nifedipine. The presence of specific receptors for ET on glomerular mesangial cells suggests a major role for this peptide in the regulation of glomerular filtration rate.
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U2 - 10.1016/0006-291X(89)92667-3
DO - 10.1016/0006-291X(89)92667-3
M3 - Article
C2 - 2544174
AN - SCOPUS:0024384465
SN - 0006-291X
VL - 161
SP - 776
EP - 781
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -