TY - JOUR
T1 - Heterogeneity of metallo and serine extracellular proteinases in oral clinical isolates of Candida albicans in HIV-positive and healthy children from Rio de Janeiro, Brazil
AU - Costa, Edja Maria Melo De Brito
AU - Dos Santos, André Luis Souza
AU - Cardoso, Abel Silveira
AU - Portela, Maristela Barbosa
AU - Abreu, Celina Monteiro
AU - Alviano, Celuta Sales
AU - Hagler, Allen Norton
AU - Soares, Rosangela Maria De Araújo
N1 - Funding Information:
We wish to thank Ricardo Ramos Mendonça Filho for the useful critical English review. This work was performed with the financial support by Financiadora de Estudos e Projetos (FINEP), Conselho Nacional de Desenvolvimento Cientı́fico e Tecnológico (CNPq), Programa de Apoio a Núcleos de Excelência (PRONEX) and Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ).
PY - 2003/9/22
Y1 - 2003/9/22
N2 - Candida yeasts frequently cause life-threatening systemic infections in immunocompromised hosts. In the present study, gelatin-SDS-PAGE analysis was used to characterize extracellular proteinases in 44 oral clinical isolates of Candida albicans from HIV-positive (29/50) and healthy children (15/50). Our survey indicates that these oral clinical isolates of C. albicans have complex extracellular proteolytic activity profiles, which illustrates the heterogeneity of this species. We showed four distinct proteolytic patterns composed of distinct serine (30-58 kDa) and metalloproteinase (64-95 kDa) activities, based on the inhibition profile with phenylmethylsulfonyl fluoride and 1,10-phenanthroline, respectively. This is the first report on secreted serine and metalloproteinases present in the culture supernatant fluids of C. albicans; however, we did not observe a significant correlation between proteolytic profile expressed by the C. albicans isolates from HIV-positive children and CD4+ T cell count and plasma viral load.
AB - Candida yeasts frequently cause life-threatening systemic infections in immunocompromised hosts. In the present study, gelatin-SDS-PAGE analysis was used to characterize extracellular proteinases in 44 oral clinical isolates of Candida albicans from HIV-positive (29/50) and healthy children (15/50). Our survey indicates that these oral clinical isolates of C. albicans have complex extracellular proteolytic activity profiles, which illustrates the heterogeneity of this species. We showed four distinct proteolytic patterns composed of distinct serine (30-58 kDa) and metalloproteinase (64-95 kDa) activities, based on the inhibition profile with phenylmethylsulfonyl fluoride and 1,10-phenanthroline, respectively. This is the first report on secreted serine and metalloproteinases present in the culture supernatant fluids of C. albicans; however, we did not observe a significant correlation between proteolytic profile expressed by the C. albicans isolates from HIV-positive children and CD4+ T cell count and plasma viral load.
KW - CD4 T cell
KW - Candida albicans
KW - Extracellular proteolytic activity
KW - HIV-infected child
KW - Oral clinical isolate
KW - Serine and metalloproteinase
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U2 - 10.1016/S0928-8244(03)00145-7
DO - 10.1016/S0928-8244(03)00145-7
M3 - Article
C2 - 13129652
AN - SCOPUS:0142090933
SN - 0928-8244
VL - 38
SP - 173
EP - 180
JO - FEMS Immunology and Medical Microbiology
JF - FEMS Immunology and Medical Microbiology
IS - 2
ER -