Heterogeneity of human basophils and mast cells in response to muscle relaxants

C. Stellato; A. de Paulis; G. de Crescenzo; F. Tatangelo; O. Rickler; G. Marone

doi:10.1007/BF01997331

Heterogeneity of human basophils and mast cells in response to muscle relaxants

C. Stellato, A. de Paulis, G. de Crescenzo, F. Tatangelo, O. Rickler, G. Marone

Research output: Contribution to journal › Article › peer-review

Abstract

We investigated the in vitro effects of increasing concentrations (10^-5-10^-3M) of four muscle relaxants (succinylcholine, d-tubocurarine, vecuronium and atracurium) on histamine release (HR) from human peripheral blood basophils and mast cells isolated from lung parenchyma (HLMC) and skin tissues (HSMC). Basophils released less than 5% of their histamine content when incubated with any one of the muscle relaxants. In contrast, mast cells showed a marked heterogeneity in their response. Succinylcholine did not induce HR from any type of mast cell, and only high concentrations of d-tubocurarine (10^-3M) caused HR from HSMC and HLMC. Vecuronium concentration-dependently induced HR from HLMC and HSMC. Atracurium concentration-dependently caused marked HR from HLMC and HSMC up to a maximum of 46.2±15.1% and 30.6±6.0%, respectively. From both HLMC and HSMC HR caused by atracurium and vecuronium was extremely rapid (t_1/2

Original language	English (US)
Journal	Agents and Actions
Volume	36
Issue number	2 Supplement
DOIs	https://doi.org/10.1007/BF01997331
State	Published - Jun 1992
Externally published	Yes

ASJC Scopus subject areas

Toxicology
Pharmacology (medical)

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title = "Heterogeneity of human basophils and mast cells in response to muscle relaxants",

abstract = "We investigated the in vitro effects of increasing concentrations (10-5-10-3M) of four muscle relaxants (succinylcholine, d-tubocurarine, vecuronium and atracurium) on histamine release (HR) from human peripheral blood basophils and mast cells isolated from lung parenchyma (HLMC) and skin tissues (HSMC). Basophils released less than 5% of their histamine content when incubated with any one of the muscle relaxants. In contrast, mast cells showed a marked heterogeneity in their response. Succinylcholine did not induce HR from any type of mast cell, and only high concentrations of d-tubocurarine (10-3M) caused HR from HSMC and HLMC. Vecuronium concentration-dependently induced HR from HLMC and HSMC. Atracurium concentration-dependently caused marked HR from HLMC and HSMC up to a maximum of 46.2±15.1% and 30.6±6.0%, respectively. From both HLMC and HSMC HR caused by atracurium and vecuronium was extremely rapid (t1/2",

author = "C. Stellato and {de Paulis}, A. and {de Crescenzo}, G. and F. Tatangelo and O. Rickler and G. Marone",

year = "1992",

month = jun,

doi = "10.1007/BF01997331",

language = "English (US)",

volume = "36",

journal = "Agents and Actions",

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number = "2 Supplement",

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T1 - Heterogeneity of human basophils and mast cells in response to muscle relaxants

AU - Stellato, C.

AU - de Paulis, A.

AU - de Crescenzo, G.

AU - Tatangelo, F.

AU - Rickler, O.

AU - Marone, G.

PY - 1992/6

Y1 - 1992/6

N2 - We investigated the in vitro effects of increasing concentrations (10-5-10-3M) of four muscle relaxants (succinylcholine, d-tubocurarine, vecuronium and atracurium) on histamine release (HR) from human peripheral blood basophils and mast cells isolated from lung parenchyma (HLMC) and skin tissues (HSMC). Basophils released less than 5% of their histamine content when incubated with any one of the muscle relaxants. In contrast, mast cells showed a marked heterogeneity in their response. Succinylcholine did not induce HR from any type of mast cell, and only high concentrations of d-tubocurarine (10-3M) caused HR from HSMC and HLMC. Vecuronium concentration-dependently induced HR from HLMC and HSMC. Atracurium concentration-dependently caused marked HR from HLMC and HSMC up to a maximum of 46.2±15.1% and 30.6±6.0%, respectively. From both HLMC and HSMC HR caused by atracurium and vecuronium was extremely rapid (t1/2

AB - We investigated the in vitro effects of increasing concentrations (10-5-10-3M) of four muscle relaxants (succinylcholine, d-tubocurarine, vecuronium and atracurium) on histamine release (HR) from human peripheral blood basophils and mast cells isolated from lung parenchyma (HLMC) and skin tissues (HSMC). Basophils released less than 5% of their histamine content when incubated with any one of the muscle relaxants. In contrast, mast cells showed a marked heterogeneity in their response. Succinylcholine did not induce HR from any type of mast cell, and only high concentrations of d-tubocurarine (10-3M) caused HR from HSMC and HLMC. Vecuronium concentration-dependently induced HR from HLMC and HSMC. Atracurium concentration-dependently caused marked HR from HLMC and HSMC up to a maximum of 46.2±15.1% and 30.6±6.0%, respectively. From both HLMC and HSMC HR caused by atracurium and vecuronium was extremely rapid (t1/2

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DO - 10.1007/BF01997331

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JO - Agents and Actions

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IS - 2 Supplement

ER -

Heterogeneity of human basophils and mast cells in response to muscle relaxants

Abstract

ASJC Scopus subject areas

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