TY - JOUR
T1 - Herpes simplex virus type II is not a cofactor to human papillomavirus in cancer of the uterine cervix
AU - Tran-Thanh, Danh
AU - Provencher, Diane
AU - Koushik, Anita
AU - Duarte-Franco, Eliane
AU - Kessous, Allégria
AU - Drouin, Pierre
AU - Wheeler, Cosette M.
AU - Dubuc-Lissoir, Josée
AU - Gauthier, Philippe
AU - Allaire, Guy
AU - Vauclair, René
AU - DiPaolo, Joseph A.
AU - Gravitt, Patty
AU - Franco, Eduardo
AU - Coutlée, Francois
N1 - Funding Information:
Supported by the Fonds de Recherche en Santé du Québec (FRSQ).
PY - 2003/1/1
Y1 - 2003/1/1
N2 - OBJECTIVE: Cells that were cotransfected with herpes simplex virus-16 and the herpes simplex virus type 2 Xho-2 DNA induce tumors in nude mice. In a cross-sectional study, we investigated the role of herpes simplex virus type 2 as a cofactor to human papillomavirus in cervical cancer. STUDY DESIGN: Cervical cells that were obtained with an endocervical Cytobrush brush (Medscand) from 439 women (50 women with cancer lesions, 65 women with high-grade squamous intraepithelial lesions, 80 women with low-grade squamous intraepithelial lesions, 244 healthy subjects) and DNA that was extracted from 150 cervical cancer biopsy specimens were analyzed with polymerase chain reaction for herpes simplex virus type 2 Xho-2 and Bgl IIC transforming DNA sequences. RESULTS: All 439 cervical samples and 150 cervical cancer biopsy specimens tested negative for herpes simplex virus type 2 Xho-2 and Bgl IIC DNA by polymerase chain reaction. Overall, none of 200 samples (0%) from women with invasive cervical cancer contained herpes simplex virus type 2 Xho-2 or Bgl IIC DNA (95% Cl, 0.0-1.8). CONCLUSION: Although herpes simplex virus type 2 BglIIN transforms epithelial cells in vitro, it was not detected in cervical cancer specimens.
AB - OBJECTIVE: Cells that were cotransfected with herpes simplex virus-16 and the herpes simplex virus type 2 Xho-2 DNA induce tumors in nude mice. In a cross-sectional study, we investigated the role of herpes simplex virus type 2 as a cofactor to human papillomavirus in cervical cancer. STUDY DESIGN: Cervical cells that were obtained with an endocervical Cytobrush brush (Medscand) from 439 women (50 women with cancer lesions, 65 women with high-grade squamous intraepithelial lesions, 80 women with low-grade squamous intraepithelial lesions, 244 healthy subjects) and DNA that was extracted from 150 cervical cancer biopsy specimens were analyzed with polymerase chain reaction for herpes simplex virus type 2 Xho-2 and Bgl IIC transforming DNA sequences. RESULTS: All 439 cervical samples and 150 cervical cancer biopsy specimens tested negative for herpes simplex virus type 2 Xho-2 and Bgl IIC DNA by polymerase chain reaction. Overall, none of 200 samples (0%) from women with invasive cervical cancer contained herpes simplex virus type 2 Xho-2 or Bgl IIC DNA (95% Cl, 0.0-1.8). CONCLUSION: Although herpes simplex virus type 2 BglIIN transforms epithelial cells in vitro, it was not detected in cervical cancer specimens.
KW - Cervical cancer
KW - Chlamydia trachomatis
KW - Herpes simplex virus-2
KW - Human papillomavirus
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U2 - 10.1067/mob.2003.66
DO - 10.1067/mob.2003.66
M3 - Article
C2 - 12548206
AN - SCOPUS:0037249260
SN - 0002-9378
VL - 188
SP - 129
EP - 134
JO - American journal of obstetrics and gynecology
JF - American journal of obstetrics and gynecology
IS - 1
ER -