Heme oxygenase-1 induction by hemin protects against gut ischemia/reperfusion injury

B. O. Attuwaybi; R. A. Kozar; S. D. Moore-Olufemi; N. Sato; H. T. Hassoun; N. W. Weisbrodt; F. A. Moore

doi:10.1016/j.jss.2004.01.010

Heme oxygenase-1 induction by hemin protects against gut ischemia/reperfusion injury

B. O. Attuwaybi, R. A. Kozar, S. D. Moore-Olufemi, N. Sato, H. T. Hassoun, N. W. Weisbrodt, F. A. Moore

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83 Scopus citations

Abstract

Background We have shown that both intraischemic hypothermia and hypertonic saline resuscitation provide dramatic protection against gut ischemia/reperfusion (I/R) injury that is in part mediated by heme oxygenase-1 (HO-1). We therefore hypothesized that induction of HO-1 by hemin would lessen damage and improve function after gut I/R. Materials and methods Male Sprague-Dawley rats were treated with 50 μmol/kg hemin (HO-1 inducer ferric protoporphyrin IX chloride) sq or vehicle 2 h before superior mesenteric artery occlusion for 60 min or sham laparotomy. After 6 h of reperfusion, transit was determined by quantitation of percentage of tracer in 10 equal segments of small intestine 30 min following injection into the duodenum (expressed as mean geometric center). Ileum was harvested for assessment of mucosal histologic injury (Chiu score 0-5 by blinded observer), myeloperoxidase activity (MPO, index of inflammation), and HO-1 protein expression. Results Hemin treatment was associated with increased HO-1 protein expression, lessened mucosal injury, decreased MPO activity, and improved intestinal transit following gut I/R. Conclusion These data corroborate that HO-1 plays an important role in protecting the gut against I/R-induced injury.

Original language	English (US)
Pages (from-to)	53-57
Number of pages	5
Journal	Journal of Surgical Research
Volume	118
Issue number	1
DOIs	https://doi.org/10.1016/j.jss.2004.01.010
State	Published - May 1 2004
Externally published	Yes

Keywords

Heme oxygenase-1
Hemin
Intestinal transit
Ischemia/reperfusion
Superior mesenteric artery occlusion

ASJC Scopus subject areas

Surgery

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10.1016/j.jss.2004.01.010

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title = "Heme oxygenase-1 induction by hemin protects against gut ischemia/reperfusion injury",

abstract = "Background We have shown that both intraischemic hypothermia and hypertonic saline resuscitation provide dramatic protection against gut ischemia/reperfusion (I/R) injury that is in part mediated by heme oxygenase-1 (HO-1). We therefore hypothesized that induction of HO-1 by hemin would lessen damage and improve function after gut I/R. Materials and methods Male Sprague-Dawley rats were treated with 50 μmol/kg hemin (HO-1 inducer ferric protoporphyrin IX chloride) sq or vehicle 2 h before superior mesenteric artery occlusion for 60 min or sham laparotomy. After 6 h of reperfusion, transit was determined by quantitation of percentage of tracer in 10 equal segments of small intestine 30 min following injection into the duodenum (expressed as mean geometric center). Ileum was harvested for assessment of mucosal histologic injury (Chiu score 0-5 by blinded observer), myeloperoxidase activity (MPO, index of inflammation), and HO-1 protein expression. Results Hemin treatment was associated with increased HO-1 protein expression, lessened mucosal injury, decreased MPO activity, and improved intestinal transit following gut I/R. Conclusion These data corroborate that HO-1 plays an important role in protecting the gut against I/R-induced injury.",

keywords = "Heme oxygenase-1, Hemin, Intestinal transit, Ischemia/reperfusion, Superior mesenteric artery occlusion",

author = "Attuwaybi, {B. O.} and Kozar, {R. A.} and Moore-Olufemi, {S. D.} and N. Sato and Hassoun, {H. T.} and Weisbrodt, {N. W.} and Moore, {F. A.}",

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doi = "10.1016/j.jss.2004.01.010",

language = "English (US)",

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T1 - Heme oxygenase-1 induction by hemin protects against gut ischemia/reperfusion injury

AU - Attuwaybi, B. O.

AU - Kozar, R. A.

AU - Moore-Olufemi, S. D.

AU - Sato, N.

AU - Hassoun, H. T.

AU - Weisbrodt, N. W.

AU - Moore, F. A.

PY - 2004/5/1

Y1 - 2004/5/1

N2 - Background We have shown that both intraischemic hypothermia and hypertonic saline resuscitation provide dramatic protection against gut ischemia/reperfusion (I/R) injury that is in part mediated by heme oxygenase-1 (HO-1). We therefore hypothesized that induction of HO-1 by hemin would lessen damage and improve function after gut I/R. Materials and methods Male Sprague-Dawley rats were treated with 50 μmol/kg hemin (HO-1 inducer ferric protoporphyrin IX chloride) sq or vehicle 2 h before superior mesenteric artery occlusion for 60 min or sham laparotomy. After 6 h of reperfusion, transit was determined by quantitation of percentage of tracer in 10 equal segments of small intestine 30 min following injection into the duodenum (expressed as mean geometric center). Ileum was harvested for assessment of mucosal histologic injury (Chiu score 0-5 by blinded observer), myeloperoxidase activity (MPO, index of inflammation), and HO-1 protein expression. Results Hemin treatment was associated with increased HO-1 protein expression, lessened mucosal injury, decreased MPO activity, and improved intestinal transit following gut I/R. Conclusion These data corroborate that HO-1 plays an important role in protecting the gut against I/R-induced injury.

AB - Background We have shown that both intraischemic hypothermia and hypertonic saline resuscitation provide dramatic protection against gut ischemia/reperfusion (I/R) injury that is in part mediated by heme oxygenase-1 (HO-1). We therefore hypothesized that induction of HO-1 by hemin would lessen damage and improve function after gut I/R. Materials and methods Male Sprague-Dawley rats were treated with 50 μmol/kg hemin (HO-1 inducer ferric protoporphyrin IX chloride) sq or vehicle 2 h before superior mesenteric artery occlusion for 60 min or sham laparotomy. After 6 h of reperfusion, transit was determined by quantitation of percentage of tracer in 10 equal segments of small intestine 30 min following injection into the duodenum (expressed as mean geometric center). Ileum was harvested for assessment of mucosal histologic injury (Chiu score 0-5 by blinded observer), myeloperoxidase activity (MPO, index of inflammation), and HO-1 protein expression. Results Hemin treatment was associated with increased HO-1 protein expression, lessened mucosal injury, decreased MPO activity, and improved intestinal transit following gut I/R. Conclusion These data corroborate that HO-1 plays an important role in protecting the gut against I/R-induced injury.

KW - Heme oxygenase-1

KW - Hemin

KW - Intestinal transit

KW - Ischemia/reperfusion

KW - Superior mesenteric artery occlusion

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Heme oxygenase-1 induction by hemin protects against gut ischemia/reperfusion injury

Abstract

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