TY - CHAP
T1 - Hemagglutination inhibition assay
AU - Spackman, Erica
AU - Sitaras, Ioannis
N1 - Publisher Copyright:
© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020.
PY - 2020
Y1 - 2020
N2 - The hemagglutination inhibition (HI) assay for influenza A virus has been used since the 1940s. The assay may be utilized to detect or quantify antibodies to influenza A viruses and can be used to characterize differences in antigenic reactivity between influenza isolates. In addition, data from HI assays are routinely used for antigenic cartography, influenza virus surveillance, epidemiology, and vaccine-seed strain selection. For antibody quantification, the HI assay is a fast and inexpensive method; other than a source of red blood cells, no expensive or unusual lab equipment is needed, and results can be obtained within a few hours. Historically, the HI assay has also served as a primary method of subtype identification and is still used widely. However, as gene sequencing technology has evolved to be cheaper and faster, it is replacing the HI assay for this purpose.
AB - The hemagglutination inhibition (HI) assay for influenza A virus has been used since the 1940s. The assay may be utilized to detect or quantify antibodies to influenza A viruses and can be used to characterize differences in antigenic reactivity between influenza isolates. In addition, data from HI assays are routinely used for antigenic cartography, influenza virus surveillance, epidemiology, and vaccine-seed strain selection. For antibody quantification, the HI assay is a fast and inexpensive method; other than a source of red blood cells, no expensive or unusual lab equipment is needed, and results can be obtained within a few hours. Historically, the HI assay has also served as a primary method of subtype identification and is still used widely. However, as gene sequencing technology has evolved to be cheaper and faster, it is replacing the HI assay for this purpose.
KW - Antigenic characterization
KW - Hemagglutination
KW - Hemagglutination inhibition
KW - Influenza subtype identification
KW - Type A influenza
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U2 - 10.1007/978-1-0716-0346-8_2
DO - 10.1007/978-1-0716-0346-8_2
M3 - Chapter
C2 - 32170677
AN - SCOPUS:85082096524
T3 - Methods in Molecular Biology
SP - 11
EP - 28
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -