Helper T-lymphocyte count: TRAx CD4 test kit versus conventional flow cytometry

Alfred J. Saah, Catherine Spruill, Donald R. Hoover, Rebecca Prevots, Ellen Taylor, Joseph B. Margolick, David Vlahov

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Background. - We evaluated a newly developed enzyme-linked immunosorbent assay system for measuring helper T-lymphocyte count. Methods. - Data from 111 human immunodeficiency virus-infected injection drug users in a cohort study were analyzed by flow cytometry and independent duplicate runs of the TRAx enzyme-linked immunosorbent assay. Results. - The mean helper T-cell counts were 470, 480, and 506 per microliter by flow cytometry and TRAx runs 1 and 2, respectively. The correlation coefficients for TRAx runs 1 and 2 with the flow cytometry results as the dependent variable were .93 and .91, respectively. A cross-tabulation of the enzyme-linked immunosorbent assay helper T-lymphocyte counts with flow cytometry counts showed agreement of 71% and 76% when the flow count was between 201 and 500, and 88% and 90% when it was greater than 500 cells per microliter. In those samples with 200 or fewer helper T cells, agreement was 73% and 41% for each TRAx run. Conclusions. - The TRAx enzyme-linked immunosorbent assay system is an acceptable method for measuring helper T-lymphocyte count, but should be recalibrated for better performance at helper T-cell counts below 200 per microliter.

Original languageEnglish (US)
Pages (from-to)960-962
Number of pages3
JournalArchives of Pathology and Laboratory Medicine
Volume121
Issue number9
StatePublished - Sep 1997

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

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