TY - JOUR
T1 - Helper T-lymphocyte count
T2 - TRAx CD4 test kit versus conventional flow cytometry
AU - Saah, Alfred J.
AU - Spruill, Catherine
AU - Hoover, Donald R.
AU - Prevots, Rebecca
AU - Taylor, Ellen
AU - Margolick, Joseph B.
AU - Vlahov, David
PY - 1997/9
Y1 - 1997/9
N2 - Background. - We evaluated a newly developed enzyme-linked immunosorbent assay system for measuring helper T-lymphocyte count. Methods. - Data from 111 human immunodeficiency virus-infected injection drug users in a cohort study were analyzed by flow cytometry and independent duplicate runs of the TRAx enzyme-linked immunosorbent assay. Results. - The mean helper T-cell counts were 470, 480, and 506 per microliter by flow cytometry and TRAx runs 1 and 2, respectively. The correlation coefficients for TRAx runs 1 and 2 with the flow cytometry results as the dependent variable were .93 and .91, respectively. A cross-tabulation of the enzyme-linked immunosorbent assay helper T-lymphocyte counts with flow cytometry counts showed agreement of 71% and 76% when the flow count was between 201 and 500, and 88% and 90% when it was greater than 500 cells per microliter. In those samples with 200 or fewer helper T cells, agreement was 73% and 41% for each TRAx run. Conclusions. - The TRAx enzyme-linked immunosorbent assay system is an acceptable method for measuring helper T-lymphocyte count, but should be recalibrated for better performance at helper T-cell counts below 200 per microliter.
AB - Background. - We evaluated a newly developed enzyme-linked immunosorbent assay system for measuring helper T-lymphocyte count. Methods. - Data from 111 human immunodeficiency virus-infected injection drug users in a cohort study were analyzed by flow cytometry and independent duplicate runs of the TRAx enzyme-linked immunosorbent assay. Results. - The mean helper T-cell counts were 470, 480, and 506 per microliter by flow cytometry and TRAx runs 1 and 2, respectively. The correlation coefficients for TRAx runs 1 and 2 with the flow cytometry results as the dependent variable were .93 and .91, respectively. A cross-tabulation of the enzyme-linked immunosorbent assay helper T-lymphocyte counts with flow cytometry counts showed agreement of 71% and 76% when the flow count was between 201 and 500, and 88% and 90% when it was greater than 500 cells per microliter. In those samples with 200 or fewer helper T cells, agreement was 73% and 41% for each TRAx run. Conclusions. - The TRAx enzyme-linked immunosorbent assay system is an acceptable method for measuring helper T-lymphocyte count, but should be recalibrated for better performance at helper T-cell counts below 200 per microliter.
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M3 - Article
C2 - 9302928
AN - SCOPUS:0031467969
SN - 0003-9985
VL - 121
SP - 960
EP - 962
JO - Archives of Pathology and Laboratory Medicine
JF - Archives of Pathology and Laboratory Medicine
IS - 9
ER -