Helicobacter pylori promotes hepatic fibrosis in the animal model

Moon Jung Goo, Mi Ran Ki, Hye Rim Lee, Hai Jie Yang, Dong Wei Yuan, Il Hwa Hong, Jin Kyu Park, Kyung Sook Hong, Jung Youn Han, Ok Kyung Hwang, Dong Hwan Kim, Sun Hee Do, Ronald D. Cohn, Kyu Shik Jeong

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Helicobacter pylori infection has been reported to be very common in patients with chronic liver diseases, including cirrhosis. To elucidate the pathological effect of H. pylori infection on the progression of hepatic fibrosis, C57BL/6 mice and Sprague-Dawley rats were orally inoculated with H. pylori, and hepatic fibrosis was induced with carbon tetrachloride (CCl 4) administration. We observed the histopathological changes and the presence of H. pylori genes by PCR in the liver. Significant increase in the fibrotic score as well as in serum alanine aminotransferase and aspartate aminotransferase levels was shown in the CCl 4 H. pylori group compared with that in the CCl 4-treated group. Compared with the CCl 4-treated group, α-smooth muscle actin and transforming growth factor-Β1 were enhanced; however, senescence marker protein-30, a multifunctional protein protecting hepatocytes against oxidative stress and apoptosis, was suppressed in the CCl 4 H. pylori group. The 16S rRNA (400 bp) was demonstrated by PCR for H. pylori genes from genomic DNA extracted from the liver, and H. pylori-infected mice showed 93.8% (15 of 16) seropositivity by contrast with seronegativity in all H. pylori-noninfected mice. In addition, immunohistochemical study against H. pylori showed positive antigen fragments in the liver of the infected groups. Consequently, our data suggest that H. pylori infection could be an important contributing infectious factor to the development of liver cirrhosis.

Original languageEnglish (US)
Pages (from-to)1291-1303
Number of pages13
JournalLaboratory Investigation
Issue number11
StatePublished - Nov 2009
Externally publishedYes


  • 16S rRNA
  • Helicobacter pylori
  • Hepatic fibrosis
  • Liver
  • Senescence marker protein 30

ASJC Scopus subject areas

  • Medicine(all)


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