HBV mutations in untreated HIV-HBV co-infection using genomic length sequencing

Jennifer Audsley; Margaret Littlejohn; Lilly Yuen; Joe Sasadeusz; Anna Ayres; Christopher Desmond; Tim Spelman; George Lau; Gail V. Matthews; Anchalee Avihingsanon; Eric Seaberg; Frances Philp; Melissa Saulynas; Kiat Ruxrungtham; Gregory J. Dore; Stephen A. Locarnini; Chloe L. Thio; Sharon R. Lewin; Peter A. Revill

doi:10.1016/j.virol.2010.06.038

HBV mutations in untreated HIV-HBV co-infection using genomic length sequencing

Jennifer Audsley, Margaret Littlejohn, Lilly Yuen, Joe Sasadeusz, Anna Ayres, Christopher Desmond, Tim Spelman, George Lau, Gail V. Matthews, Anchalee Avihingsanon, Eric Seaberg, Frances Philp, Melissa Saulynas, Kiat Ruxrungtham, Gregory J. Dore, Stephen A. Locarnini, Chloe L. Thio, Sharon R. Lewin, Peter A. Revill

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

HIV infection has a significant impact on the natural progression of hepatitis B virus (HBV) related liver disease. In HIV-HBV co-infected patients, little is known about mutations in the HBV genome, which can influence severity of liver disease. The aim of this study was to characterize and to determine the frequency of known clinically significant mutations in the HBV genomes from HIV-HBV co-infected patients and from HBV mono-infected patients. To accomplish this, genomic length HBV sequencing was performed in highly-active anti-retroviral therapy (HAART)-naïve HIV-HBV co-infected patients (n=74) and in anti-HBV therapy-naïve HBV mono-infected patients (n=55).The frequency of HBV mutations differed between the co-infected and mono-infected patients when comparing patients with the same genotype. BCP mutations A1762T and G1764A were significantly more frequent in HBV genotype C mono-infection and the -1G frameshift was significantly more frequent in co-infection and was only observed in HBV genotype A co-infection. PreS2 deletions were observed more frequently in the setting of co-infection. Further work is needed to determine if these mutational patterns influence the differences in liver disease progression in HIV-HBV co-infected and HBV mono-infected patients.

Original language	English (US)
Pages (from-to)	539-547
Number of pages	9
Journal	Virology
Volume	405
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2010.06.038
State	Published - Sep 30 2010

Keywords

Genomic length sequencing
HIV-HBV co-infection
PreHAART

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2010.06.038

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Audsley, J., Littlejohn, M., Yuen, L., Sasadeusz, J., Ayres, A., Desmond, C., Spelman, T., Lau, G., Matthews, G. V., Avihingsanon, A., Seaberg, E., Philp, F., Saulynas, M., Ruxrungtham, K., Dore, G. J., Locarnini, S. A., Thio, C. L., Lewin, S. R., & Revill, P. A. (2010). HBV mutations in untreated HIV-HBV co-infection using genomic length sequencing. Virology, 405(2), 539-547. https://doi.org/10.1016/j.virol.2010.06.038

Audsley, J, Littlejohn, M, Yuen, L, Sasadeusz, J, Ayres, A, Desmond, C, Spelman, T, Lau, G, Matthews, GV, Avihingsanon, A, Seaberg, E, Philp, F, Saulynas, M, Ruxrungtham, K, Dore, GJ, Locarnini, SA, Thio, CL, Lewin, SR & Revill, PA 2010, 'HBV mutations in untreated HIV-HBV co-infection using genomic length sequencing', Virology, vol. 405, no. 2, pp. 539-547. https://doi.org/10.1016/j.virol.2010.06.038

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title = "HBV mutations in untreated HIV-HBV co-infection using genomic length sequencing",

abstract = "HIV infection has a significant impact on the natural progression of hepatitis B virus (HBV) related liver disease. In HIV-HBV co-infected patients, little is known about mutations in the HBV genome, which can influence severity of liver disease. The aim of this study was to characterize and to determine the frequency of known clinically significant mutations in the HBV genomes from HIV-HBV co-infected patients and from HBV mono-infected patients. To accomplish this, genomic length HBV sequencing was performed in highly-active anti-retroviral therapy (HAART)-na{\"i}ve HIV-HBV co-infected patients (n=74) and in anti-HBV therapy-na{\"i}ve HBV mono-infected patients (n=55).The frequency of HBV mutations differed between the co-infected and mono-infected patients when comparing patients with the same genotype. BCP mutations A1762T and G1764A were significantly more frequent in HBV genotype C mono-infection and the -1G frameshift was significantly more frequent in co-infection and was only observed in HBV genotype A co-infection. PreS2 deletions were observed more frequently in the setting of co-infection. Further work is needed to determine if these mutational patterns influence the differences in liver disease progression in HIV-HBV co-infected and HBV mono-infected patients.",

keywords = "Genomic length sequencing, HIV-HBV co-infection, PreHAART",

author = "Jennifer Audsley and Margaret Littlejohn and Lilly Yuen and Joe Sasadeusz and Anna Ayres and Christopher Desmond and Tim Spelman and George Lau and Matthews, {Gail V.} and Anchalee Avihingsanon and Eric Seaberg and Frances Philp and Melissa Saulynas and Kiat Ruxrungtham and Dore, {Gregory J.} and Locarnini, {Stephen A.} and Thio, {Chloe L.} and Lewin, {Sharon R.} and Revill, {Peter A.}",

note = "Funding Information: We thank Professor Kit Fairley, Melbourne Sexual Health Centre, Melbourne Australia; Dr Robert Finlayson, Taylor Square Private Clinic, Darlinghurst Australia; Professor David Cooper, St Vincent's Hospital, Sydney Australia and Ms Pip Marks, NCHECR, Sydney Australia for their assistance in participant recruitment. We thank Dr. Stephane Hue for helpful advice with the phylogenetic analysis. We acknowledge funding from the National Institute of Health RO1 A1060449 . Some of the data in this manuscript were collected by the Multicenter AIDS Cohort Study (MACS) with centers (Principal Investigators) at The Johns Hopkins Bloomberg School of Public Health (Joseph B. Margolick, Lisa P. Jacobson), Howard Brown Health Center, Feinberg School of Medicine, Northwestern University, and Cook County Bureau of Health Services (John P. Phair, Steven M. Wolinsky), University of California, Los Angeles (Roger Detels), and University of Pittsburgh (Charles R. Rinaldo). The MACS is funded by the National Institute of Allergy and Infectious Diseases, with additional supplemental funding from the National Cancer Institute. UO1-AI-35042, UL1-RR025005 (GCRC), UO1-AI-35043, UO1-AI-35039, UO1-AI-35040, UO1-AI-35041. Website located at http://www.statepi.jhsph.edu/macs/macs.html .",

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doi = "10.1016/j.virol.2010.06.038",

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AU - Audsley, Jennifer

AU - Littlejohn, Margaret

AU - Yuen, Lilly

AU - Sasadeusz, Joe

AU - Ayres, Anna

AU - Desmond, Christopher

AU - Spelman, Tim

AU - Lau, George

AU - Matthews, Gail V.

AU - Avihingsanon, Anchalee

AU - Seaberg, Eric

AU - Philp, Frances

AU - Saulynas, Melissa

AU - Ruxrungtham, Kiat

AU - Dore, Gregory J.

AU - Locarnini, Stephen A.

AU - Thio, Chloe L.

AU - Lewin, Sharon R.

AU - Revill, Peter A.

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N2 - HIV infection has a significant impact on the natural progression of hepatitis B virus (HBV) related liver disease. In HIV-HBV co-infected patients, little is known about mutations in the HBV genome, which can influence severity of liver disease. The aim of this study was to characterize and to determine the frequency of known clinically significant mutations in the HBV genomes from HIV-HBV co-infected patients and from HBV mono-infected patients. To accomplish this, genomic length HBV sequencing was performed in highly-active anti-retroviral therapy (HAART)-naïve HIV-HBV co-infected patients (n=74) and in anti-HBV therapy-naïve HBV mono-infected patients (n=55).The frequency of HBV mutations differed between the co-infected and mono-infected patients when comparing patients with the same genotype. BCP mutations A1762T and G1764A were significantly more frequent in HBV genotype C mono-infection and the -1G frameshift was significantly more frequent in co-infection and was only observed in HBV genotype A co-infection. PreS2 deletions were observed more frequently in the setting of co-infection. Further work is needed to determine if these mutational patterns influence the differences in liver disease progression in HIV-HBV co-infected and HBV mono-infected patients.

AB - HIV infection has a significant impact on the natural progression of hepatitis B virus (HBV) related liver disease. In HIV-HBV co-infected patients, little is known about mutations in the HBV genome, which can influence severity of liver disease. The aim of this study was to characterize and to determine the frequency of known clinically significant mutations in the HBV genomes from HIV-HBV co-infected patients and from HBV mono-infected patients. To accomplish this, genomic length HBV sequencing was performed in highly-active anti-retroviral therapy (HAART)-naïve HIV-HBV co-infected patients (n=74) and in anti-HBV therapy-naïve HBV mono-infected patients (n=55).The frequency of HBV mutations differed between the co-infected and mono-infected patients when comparing patients with the same genotype. BCP mutations A1762T and G1764A were significantly more frequent in HBV genotype C mono-infection and the -1G frameshift was significantly more frequent in co-infection and was only observed in HBV genotype A co-infection. PreS2 deletions were observed more frequently in the setting of co-infection. Further work is needed to determine if these mutational patterns influence the differences in liver disease progression in HIV-HBV co-infected and HBV mono-infected patients.

KW - Genomic length sequencing

KW - HIV-HBV co-infection

KW - PreHAART

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HBV mutations in untreated HIV-HBV co-infection using genomic length sequencing

Abstract

Keywords

ASJC Scopus subject areas

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