TY - JOUR
T1 - Guidance Document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0)
AU - Pamies, David
AU - Leist, Marcel
AU - Coecke, Sandra
AU - Bowe, Gerard
AU - Allen, David G.
AU - Gstraunthaler, Gerhard
AU - Bal-Price, Anna
AU - Pistollato, Francesca
AU - de Vries, Rob B.M.
AU - Hogberg, Helena T.
AU - Hartung, Thomas
AU - Stacey, Glyn
N1 - Publisher Copyright:
© The Authors, 2022.
PY - 2022
Y1 - 2022
N2 - Good Cell and Tissue Culture Practice (GCCP) 2.0 is an updated guidance document from GCCP 1.0 (published by ECVAM in 2005), which was developed for practical use in the laboratory to assure the reproducibility of in vitro (cell-based) work. The update in the guidance was essential as cell models have advanced dramatically to more complex culture systems and need more comprehensive quality management to ensure reproducibility and high-quality scientific data. This document describes six main principles to consider when performing cell culture including characterization and maintenance of essential characteristics, quality management, documentation and reporting, safety, education and training, and ethics. The document does not intend to impose detailed procedures but to describe potential quality issues. It is foreseen that the document will require further updates as the science and technologies evolve over time.
AB - Good Cell and Tissue Culture Practice (GCCP) 2.0 is an updated guidance document from GCCP 1.0 (published by ECVAM in 2005), which was developed for practical use in the laboratory to assure the reproducibility of in vitro (cell-based) work. The update in the guidance was essential as cell models have advanced dramatically to more complex culture systems and need more comprehensive quality management to ensure reproducibility and high-quality scientific data. This document describes six main principles to consider when performing cell culture including characterization and maintenance of essential characteristics, quality management, documentation and reporting, safety, education and training, and ethics. The document does not intend to impose detailed procedures but to describe potential quality issues. It is foreseen that the document will require further updates as the science and technologies evolve over time.
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U2 - 10.14573/altex.2111011
DO - 10.14573/altex.2111011
M3 - Article
C2 - 34882777
AN - SCOPUS:85123813001
SN - 1868-596X
VL - 39
SP - 30
EP - 70
JO - ALTEX
JF - ALTEX
IS - 1
ER -