Grp1-associated scaffold protein regulates skin homeostasis after ultraviolet irradiation

Anand Venkataraman; Daniel J. Coleman; Daniel J. Nevrivy; Tulley Long; Chrissa Kioussi; Arup K. Indra; Mark Leid

doi:10.1039/c3pp50351h

Grp1-associated scaffold protein regulates skin homeostasis after ultraviolet irradiation

Anand Venkataraman, Daniel J. Coleman, Daniel J. Nevrivy, Tulley Long, Chrissa Kioussi, Arup K. Indra, Mark Leid

Research output: Contribution to journal › Article › peer-review

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Abstract

Grp1-associated scaffold protein (Grasp), the product of a retinoic acid-induced gene in P19 embryonal carcinoma cells, is expressed primarily in brain, heart, and lung of the mouse. We report herein that Grasp transcripts are also found in mouse skin in which the Grasp gene is robustly induced following acute ultraviolet-B (UVB) exposure. Grasp^-/- mice were found to exhibit delayed epidermal proliferation and a blunted apoptotic response after acute UVB exposure. Immunohistochemical analyses revealed that the nuclear residence time of the tumor suppressor protein p53 was reduced in Grasp ^-/- mice after UVB exposure. Taken together, our results suggest that a physiological role of Grasp may be to regulate skin homeostasis after UVB exposure, potentially by influencing p53-mediated apoptotic responses in skin.

Original language	English (US)
Pages (from-to)	531-540
Number of pages	10
Journal	Photochemical and Photobiological Sciences
Volume	13
Issue number	3
DOIs	https://doi.org/10.1039/c3pp50351h
State	Published - Mar 2014
Externally published	Yes

ASJC Scopus subject areas

Physical and Theoretical Chemistry

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title = "Grp1-associated scaffold protein regulates skin homeostasis after ultraviolet irradiation",

abstract = "Grp1-associated scaffold protein (Grasp), the product of a retinoic acid-induced gene in P19 embryonal carcinoma cells, is expressed primarily in brain, heart, and lung of the mouse. We report herein that Grasp transcripts are also found in mouse skin in which the Grasp gene is robustly induced following acute ultraviolet-B (UVB) exposure. Grasp-/- mice were found to exhibit delayed epidermal proliferation and a blunted apoptotic response after acute UVB exposure. Immunohistochemical analyses revealed that the nuclear residence time of the tumor suppressor protein p53 was reduced in Grasp -/- mice after UVB exposure. Taken together, our results suggest that a physiological role of Grasp may be to regulate skin homeostasis after UVB exposure, potentially by influencing p53-mediated apoptotic responses in skin.",

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doi = "10.1039/c3pp50351h",

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AU - Venkataraman, Anand

AU - Coleman, Daniel J.

AU - Nevrivy, Daniel J.

AU - Long, Tulley

AU - Kioussi, Chrissa

AU - Indra, Arup K.

AU - Leid, Mark

PY - 2014/3

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AB - Grp1-associated scaffold protein (Grasp), the product of a retinoic acid-induced gene in P19 embryonal carcinoma cells, is expressed primarily in brain, heart, and lung of the mouse. We report herein that Grasp transcripts are also found in mouse skin in which the Grasp gene is robustly induced following acute ultraviolet-B (UVB) exposure. Grasp-/- mice were found to exhibit delayed epidermal proliferation and a blunted apoptotic response after acute UVB exposure. Immunohistochemical analyses revealed that the nuclear residence time of the tumor suppressor protein p53 was reduced in Grasp -/- mice after UVB exposure. Taken together, our results suggest that a physiological role of Grasp may be to regulate skin homeostasis after UVB exposure, potentially by influencing p53-mediated apoptotic responses in skin.

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Grp1-associated scaffold protein regulates skin homeostasis after ultraviolet irradiation

Abstract

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