TY - JOUR
T1 - Growth/differentiation factor-10
T2 - A new member of the transforming growth factor-β superfamily related to bone morphogenetic protein-3
AU - Cunningham, Noreen S.
AU - Jenkins, Nancy A.
AU - Gilbert, Debra J.
AU - Copeland, Neal G.
AU - Reddi, A. Hari
AU - Lee, Se Jin
N1 - Funding Information:
We thank Thanh Huynh, Alexandra McPherron, and Aurora Esquela for helpful discussions, M. E. Barnstead for excellent technical assistance with the mapping of GdflO, and Christine Moss for assistance in preparing the manuscript. This work was supported in part by the National Cancer Institute, DHHS, under contract N01-CO-74101 with ABL (N. A. J. and N. G. C), by SPORE grant CA-58236 from the National Cancer Institute (N. S. C. and A. H. R.), and by grants from the Edward Mallinckrodt, Jr. Foundation, the American Cancer Society (CB-l4B), and the National Institutes of Health (R01 HD30740) (S.-J. L.).
PY - 1995
Y1 - 1995
N2 - We have identified a new member of the transforming growth factor-β (TGF-β superfamily, growth/differentiation factor-10 (GDF-10), which is highly related to bone morphogenetic protein-3 (BMP-3). The nucleotide sequence of GDF-10 encodes a predicted protein of 476 amino acids with a molecular weight of approximately 52,000. The GDF-10 polypeptide contains a potential signal sequence for secretion, a putative RXXR proteolytic processing site, and a carboxy-terminal domain with considerable homology to other known members of the TGF-β superfamily. In the mature carboxy-terminal domain GDF-10 is more homologous to BMP-3 (83% amino acid sequence identity) than to any other previously identified TGF-β family member. GDF-10 also shows significant homology to BMP-3 (approximately 30% amino acid sequence identity) in the pro-region of the molecule. Based on these sequence comparisons, GDF-10 and BMP-3 define a new subgroup within the larger TGF-β superfamily. By Northern analysis, GDF-10 mRNA was detected primarily in murine uterus, adipose tissue, and brain and to a lesser extent in liver and spleen. In addition, GDF-10 mRNA was present in both neonatal and adult bone samples, with higher levels being detected in calvaria than in long bone. These results suggest that GDF10 may play multiple roles in regulating cell differentiation events, including those involved in skeletal morphogenesis. Gdf10 was mapped to the proximal region of mouse chromosome 14 close to a region known to contain a spontaneous recessive mutation that is associated with a craniofacial defect.
AB - We have identified a new member of the transforming growth factor-β (TGF-β superfamily, growth/differentiation factor-10 (GDF-10), which is highly related to bone morphogenetic protein-3 (BMP-3). The nucleotide sequence of GDF-10 encodes a predicted protein of 476 amino acids with a molecular weight of approximately 52,000. The GDF-10 polypeptide contains a potential signal sequence for secretion, a putative RXXR proteolytic processing site, and a carboxy-terminal domain with considerable homology to other known members of the TGF-β superfamily. In the mature carboxy-terminal domain GDF-10 is more homologous to BMP-3 (83% amino acid sequence identity) than to any other previously identified TGF-β family member. GDF-10 also shows significant homology to BMP-3 (approximately 30% amino acid sequence identity) in the pro-region of the molecule. Based on these sequence comparisons, GDF-10 and BMP-3 define a new subgroup within the larger TGF-β superfamily. By Northern analysis, GDF-10 mRNA was detected primarily in murine uterus, adipose tissue, and brain and to a lesser extent in liver and spleen. In addition, GDF-10 mRNA was present in both neonatal and adult bone samples, with higher levels being detected in calvaria than in long bone. These results suggest that GDF10 may play multiple roles in regulating cell differentiation events, including those involved in skeletal morphogenesis. Gdf10 was mapped to the proximal region of mouse chromosome 14 close to a region known to contain a spontaneous recessive mutation that is associated with a craniofacial defect.
KW - Bone morphogenetic protein-3
KW - Growth/differentiation factor
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U2 - 10.3109/08977199509028956
DO - 10.3109/08977199509028956
M3 - Article
C2 - 8679252
AN - SCOPUS:0029119066
SN - 0897-7194
VL - 12
SP - 99
EP - 109
JO - Growth Factors
JF - Growth Factors
IS - 2
ER -