GPQuest: A spectral library matching algorithm for site-specific assignment of tandem mass spectra to intact n-glycopeptides

Keyphrases

• Glycopeptides 100%
• Tandem Mass Spectra 100%
• Matching Algorithm 100%
• Glycosite 100%
• Specific Assignments 100%
• Spectral Library Matching 100%
• Intact Glycopeptides 87%
• Glycans 62%
• Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) 37%
• MS Spectra 37%
• Energy-resolved Collision-induced Dissociation (ER-CID) 37%
• Glycosylation Site 25%
• MS-MS 25%
• Oxonium Ion 25%
• Spectral Library 25%
• Glycan Databases 25%
• Glycoprotein 12%
• Biological Processes 12%
• N-linked 12%
• Protein Abundance 12%
• Recent Advances 12%
• Prostate Tumor 12%
• High-throughput 12%
• False Discovery Rate 12%
• Occupancy 12%
• Mass Spectrometry Methods 12%
• LNCaP Cells 12%
• Pathological Process 12%
• N-glycan 12%
• Tryptic Peptides 12%
• Precursor Ions 12%
• Glycoform 12%
• Complex Biological Samples 12%
• Site-specific Assessment 12%
• Protein Extract 12%
• Mass Difference 12%
• Complex Samples 12%
• Mass Spectrometry Instrumentation 12%
• N-linked Glycopeptides 12%
• Without Enrichment 12%

Chemistry

• Tandem Mass Spectrum 100%
• Glycopeptide 100%
• Polysaccharide 58%
• Peptide 50%
• Tandem Mass Spectrometry 41%
• Liquid Chromatography Mass Spectrometry 25%
• Collisional 25%
• Oxonium 16%
• Glycosylation 16%
• Mass Spectrometry 8%
• Glycoprotein 8%
• N-Glycan 8%

Biochemistry, Genetics and Molecular Biology

• Glycopeptide 100%
• Polysaccharide 66%
• Peptide 50%
• Tandem Mass Spectrometry 41%
• Liquid Chromatography-Mass Spectrometry 25%
• Glycosylation 16%
• Glycoprotein 8%
• Precursor 8%
• Extract 8%
• Mass Spectrometry 8%
• LNCaP 8%