Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera

Niamh Murphy; Barrie Rooney; Tapan Bhattacharyya; Omar Triana-Chavez; Anja Krueger; Stuart M. Haslam; Victoria O’Rourke; Magdalena Pańczuk; Jemima Tsang; Jack Bickford-Smith; Robert H. Gilman; Kevin Tetteh; Chris Drakeley; C. Mark Smales; Michael A. Miles

doi:10.1038/s41598-020-73390-9

Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera

Niamh Murphy, Barrie Rooney, Tapan Bhattacharyya, Omar Triana-Chavez, Anja Krueger, Stuart M. Haslam, Victoria O’Rourke, Magdalena Pańczuk, Jemima Tsang, Jack Bickford-Smith, Robert H. Gilman, Kevin Tetteh, Chris Drakeley, C. Mark Smales, Michael A. Miles

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Chagas disease is considered the most important parasitic disease in Latin America. The protozoan agent, Trypanosoma cruzi, comprises six genetic lineages, TcI-TcVI. Genotyping to link lineage(s) to severity of cardiomyopathy and gastrointestinal pathology is impeded by the sequestration and replication of T. cruzi in host tissues. We describe serology specific for TcI, the predominant lineage north of the Amazon, based on expression of recombinant trypomastigote small surface antigen (gTSSA-I) in the eukaryote Leishmania tarentolae, to allow realistic glycosylation and structure of the antigen. Sera from TcI-endemic regions recognised gTSSA-I (74/146; 50.7%), with no cross reaction with common components of gTSSA-II/V/VI recombinant antigen. Antigenicity was abolished by chemical (periodate) oxidation of gTSSA-I glycosylation but retained after heat-denaturation of conformation. Conversely, non-specific recognition of gTSSA-I by non-endemic malaria sera was abolished by heat-denaturation. TcI-specific serology facilitates investigation between lineage and diverse clinical presentations. Glycosylation cannot be ignored in the search for immunogenic antigens.

Original language	English (US)
Article number	16395
Journal	Scientific reports
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41598-020-73390-9
State	Published - Dec 1 2020

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Murphy, N., Rooney, B., Bhattacharyya, T., Triana-Chavez, O., Krueger, A., Haslam, S. M., O’Rourke, V., Pańczuk, M., Tsang, J., Bickford-Smith, J., Gilman, R. H., Tetteh, K., Drakeley, C., Smales, C. M., & Miles, M. A. (2020). Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera. Scientific reports, 10(1), Article 16395. https://doi.org/10.1038/s41598-020-73390-9

Murphy, N, Rooney, B, Bhattacharyya, T, Triana-Chavez, O, Krueger, A, Haslam, SM, O’Rourke, V, Pańczuk, M, Tsang, J, Bickford-Smith, J, Gilman, RH, Tetteh, K, Drakeley, C, Smales, CM & Miles, MA 2020, 'Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera', Scientific reports, vol. 10, no. 1, 16395. https://doi.org/10.1038/s41598-020-73390-9

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abstract = "Chagas disease is considered the most important parasitic disease in Latin America. The protozoan agent, Trypanosoma cruzi, comprises six genetic lineages, TcI-TcVI. Genotyping to link lineage(s) to severity of cardiomyopathy and gastrointestinal pathology is impeded by the sequestration and replication of T. cruzi in host tissues. We describe serology specific for TcI, the predominant lineage north of the Amazon, based on expression of recombinant trypomastigote small surface antigen (gTSSA-I) in the eukaryote Leishmania tarentolae, to allow realistic glycosylation and structure of the antigen. Sera from TcI-endemic regions recognised gTSSA-I (74/146; 50.7%), with no cross reaction with common components of gTSSA-II/V/VI recombinant antigen. Antigenicity was abolished by chemical (periodate) oxidation of gTSSA-I glycosylation but retained after heat-denaturation of conformation. Conversely, non-specific recognition of gTSSA-I by non-endemic malaria sera was abolished by heat-denaturation. TcI-specific serology facilitates investigation between lineage and diverse clinical presentations. Glycosylation cannot be ignored in the search for immunogenic antigens.",

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AU - Rooney, Barrie

AU - Bhattacharyya, Tapan

AU - Triana-Chavez, Omar

AU - Krueger, Anja

AU - Haslam, Stuart M.

AU - O’Rourke, Victoria

AU - Pańczuk, Magdalena

AU - Tsang, Jemima

AU - Bickford-Smith, Jack

AU - Gilman, Robert H.

AU - Tetteh, Kevin

AU - Drakeley, Chris

AU - Smales, C. Mark

AU - Miles, Michael A.

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Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera

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