Global synthetic-lethality analysis and yeast functional profiling

Siew Loon Ooi; Xuewen Pan; Brian D. Peyser; Ping Ye; Pamela Meluh; Daniel S. Yuan; Rafael Irizarry; Joel S. Bader; Forrest Spencer; Jef D. Boeke

doi:10.1016/j.tig.2005.11.003

Global synthetic-lethality analysis and yeast functional profiling

Siew Loon Ooi, Xuewen Pan, Brian D. Peyser, Ping Ye, Pamela Meluh, Daniel S. Yuan, Rafael Irizarry, Joel S. Bader, Forrest Spencer, Jef D. Boeke

Research output: Contribution to journal › Review article › peer-review

117 Scopus citations

Abstract

The Saccharomyces genome-deletion project created >5900 'molecularly barcoded' yeast knockout mutants (YKO mutants). The YKO mutant collections have facilitated large-scale analyses of a multitude of mutant phenotypes. For example, both synthetic genetic array (SGA) and synthetic-lethality analysis by microarray (SLAM) methods have been used for synthetic-lethality screens. Global analysis of synthetic lethality promises to identify cellular pathways that 'buffer' each other biologically. The combination of global synthetic-lethality analysis, together with global protein-protein interaction analyses, mRNA expression profiling and functional profiling will, in principle, enable construction of a cellular 'wiring diagram' that will help frame a deeper understanding of human biology and disease.

Original language	English (US)
Pages (from-to)	56-63
Number of pages	8
Journal	Trends in Genetics
Volume	22
Issue number	1
DOIs	https://doi.org/10.1016/j.tig.2005.11.003
State	Published - Jan 2006

ASJC Scopus subject areas

Genetics

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10.1016/j.tig.2005.11.003

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title = "Global synthetic-lethality analysis and yeast functional profiling",

abstract = "The Saccharomyces genome-deletion project created >5900 'molecularly barcoded' yeast knockout mutants (YKO mutants). The YKO mutant collections have facilitated large-scale analyses of a multitude of mutant phenotypes. For example, both synthetic genetic array (SGA) and synthetic-lethality analysis by microarray (SLAM) methods have been used for synthetic-lethality screens. Global analysis of synthetic lethality promises to identify cellular pathways that 'buffer' each other biologically. The combination of global synthetic-lethality analysis, together with global protein-protein interaction analyses, mRNA expression profiling and functional profiling will, in principle, enable construction of a cellular 'wiring diagram' that will help frame a deeper understanding of human biology and disease.",

author = "Ooi, {Siew Loon} and Xuewen Pan and Peyser, {Brian D.} and Ping Ye and Pamela Meluh and Yuan, {Daniel S.} and Rafael Irizarry and Bader, {Joel S.} and Forrest Spencer and Boeke, {Jef D.}",

note = "Funding Information: We thank our anonymous reviewers for their thoughtful comments that greatly improved this article. We apologize to authors whose work could not be cited owing to space limitations. Our work is supported by grants from the NIH. ",

year = "2006",

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doi = "10.1016/j.tig.2005.11.003",

language = "English (US)",

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T1 - Global synthetic-lethality analysis and yeast functional profiling

AU - Ooi, Siew Loon

AU - Pan, Xuewen

AU - Peyser, Brian D.

AU - Ye, Ping

AU - Meluh, Pamela

AU - Yuan, Daniel S.

AU - Irizarry, Rafael

AU - Bader, Joel S.

AU - Spencer, Forrest

AU - Boeke, Jef D.

N1 - Funding Information: We thank our anonymous reviewers for their thoughtful comments that greatly improved this article. We apologize to authors whose work could not be cited owing to space limitations. Our work is supported by grants from the NIH.

PY - 2006/1

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N2 - The Saccharomyces genome-deletion project created >5900 'molecularly barcoded' yeast knockout mutants (YKO mutants). The YKO mutant collections have facilitated large-scale analyses of a multitude of mutant phenotypes. For example, both synthetic genetic array (SGA) and synthetic-lethality analysis by microarray (SLAM) methods have been used for synthetic-lethality screens. Global analysis of synthetic lethality promises to identify cellular pathways that 'buffer' each other biologically. The combination of global synthetic-lethality analysis, together with global protein-protein interaction analyses, mRNA expression profiling and functional profiling will, in principle, enable construction of a cellular 'wiring diagram' that will help frame a deeper understanding of human biology and disease.

AB - The Saccharomyces genome-deletion project created >5900 'molecularly barcoded' yeast knockout mutants (YKO mutants). The YKO mutant collections have facilitated large-scale analyses of a multitude of mutant phenotypes. For example, both synthetic genetic array (SGA) and synthetic-lethality analysis by microarray (SLAM) methods have been used for synthetic-lethality screens. Global analysis of synthetic lethality promises to identify cellular pathways that 'buffer' each other biologically. The combination of global synthetic-lethality analysis, together with global protein-protein interaction analyses, mRNA expression profiling and functional profiling will, in principle, enable construction of a cellular 'wiring diagram' that will help frame a deeper understanding of human biology and disease.

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Global synthetic-lethality analysis and yeast functional profiling

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