TY - JOUR
T1 - Global knockout of ROMK potassium channel worsens cardiac ischemia-reperfusion injury but cardiomyocyte-specific knockout does not
T2 - Implications for the identity of mitoKATP
AU - Papanicolaou, Kyriakos N.
AU - Ashok, Deepthi
AU - Liu, Ting
AU - Bauer, Tyler M.
AU - Sun, Junhui
AU - Li, Zhen
AU - da Costa, Eduardo
AU - D'Orleans, Charles Crepy
AU - Nathan, Sara
AU - Lefer, David J.
AU - Murphy, Elizabeth
AU - Paolocci, Nazareno
AU - Foster, D. Brian
AU - O'Rourke, Brian
N1 - Funding Information:
This work was supported by an AHA Postdoctoral fellowship (15POST24700006) to KNP and an AHA Scientist Development Grant (12SDG12060056) to DBF. KNP also acknowledges support from NIH grant K12 HL141952. DA is supported by NIH F31 grant HL134198. Work in the lab of EM is supported by ZIA-HL002066. Work in the lab of NP is supported by R01HL136918. Work in the lab of DBF is supported by an AHA Transformational Project Award (18TPA34170575) and R01HL134821. Work in the lab of BO'R is supported by grants R01HL137259 and R01HL134821. We thank Chip Hawkins and the Johns Hopkins Transgenic core for embryo microinjections. We thank Agnes Sidor for preparing adenoviruses, Djahida Bedja for performing and analyzing echocardiograms, Guangshuo Zhu for performing and analyzing pressure-volume loops and Ophelia Rogers for performing complete blood counts.
Funding Information:
This work was supported by an AHA Postdoctoral fellowship ( 15POST24700006 ) to KNP and an AHA Scientist Development Grant ( 12SDG12060056 ) to DBF. KNP also acknowledges support from NIH grant K12 HL141952 . DA is supported by NIH F31 grant HL134198 . Work in the lab of EM is supported by ZIA-HL002066. Work in the lab of NP is supported by R01HL136918. Work in the lab of DBF is supported by an AHA Transformational Project Award ( 18TPA34170575 ) and R01HL134821 . Work in the lab of BO'R is supported by grants R01HL137259 and R01HL134821 . We thank Chip Hawkins and the Johns Hopkins Transgenic core for embryo microinjections. We thank Agnes Sidor for preparing adenoviruses, Djahida Bedja for performing and analyzing echocardiograms, Guangshuo Zhu for performing and analyzing pressure-volume loops and Ophelia Rogers for performing complete blood counts.
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/2
Y1 - 2020/2
N2 - The renal-outer-medullary‑potassium (ROMK) channel, mutated in Bartter's syndrome, regulates ion exchange in kidney, but its extra-renal functions remain unknown. Additionally, ROMK was postulated to be the pore-forming subunit of the mitochondrial ATP-sensitive K+ channel (mitoKATP), a mediator of cardioprotection. Using global and cardiomyocyte-specific knockout mice (ROMK-GKO and ROMK-CKO respectively), we characterize the effects of ROMK knockout on mitochondrial ion handling, the response to pharmacological KATP channel modulators, and ischemia/reperfusion (I/R) injury. Mitochondria from ROMK-GKO hearts exhibited a lower threshold for Ca2+-triggered permeability transition pore (mPTP) opening but normal matrix volume changes during oxidative phosphorylation. Isolated perfused ROMK-GKO hearts exhibited impaired functional recovery and increased infarct size when I/R was preceded by an ischemic preconditioning (IPC) protocol. Because ROMK-GKO mice exhibited severe renal defects and cardiac remodeling, we further characterized ROMK-CKO hearts to avoid confounding systemic effects. Mitochondria from ROMK-CKO hearts had unchanged matrix volume responses during oxidative phosphorylation and still swelled upon addition of a mitoKATP opener, but exhibited a lower threshold for mPTP opening, similar to GKO mitochondria. Nevertheless, I/R induced damage was not exacerbated in ROMK-CKO hearts, either ex vivo or in vivo. Lastly, we examined the response of ROMK-CKO hearts to ex vivo I/R injury with or without IPC and found that IPC still protected these hearts, suggesting that cardiomyocyte ROMK does not participate significantly in the cardioprotective pathway elicited by IPC. Collectively, our findings from these novel strains of mice suggest that cardiomyocyte ROMK is not a central mediator of mitoKATP function, although it can affect mPTP activation threshold.
AB - The renal-outer-medullary‑potassium (ROMK) channel, mutated in Bartter's syndrome, regulates ion exchange in kidney, but its extra-renal functions remain unknown. Additionally, ROMK was postulated to be the pore-forming subunit of the mitochondrial ATP-sensitive K+ channel (mitoKATP), a mediator of cardioprotection. Using global and cardiomyocyte-specific knockout mice (ROMK-GKO and ROMK-CKO respectively), we characterize the effects of ROMK knockout on mitochondrial ion handling, the response to pharmacological KATP channel modulators, and ischemia/reperfusion (I/R) injury. Mitochondria from ROMK-GKO hearts exhibited a lower threshold for Ca2+-triggered permeability transition pore (mPTP) opening but normal matrix volume changes during oxidative phosphorylation. Isolated perfused ROMK-GKO hearts exhibited impaired functional recovery and increased infarct size when I/R was preceded by an ischemic preconditioning (IPC) protocol. Because ROMK-GKO mice exhibited severe renal defects and cardiac remodeling, we further characterized ROMK-CKO hearts to avoid confounding systemic effects. Mitochondria from ROMK-CKO hearts had unchanged matrix volume responses during oxidative phosphorylation and still swelled upon addition of a mitoKATP opener, but exhibited a lower threshold for mPTP opening, similar to GKO mitochondria. Nevertheless, I/R induced damage was not exacerbated in ROMK-CKO hearts, either ex vivo or in vivo. Lastly, we examined the response of ROMK-CKO hearts to ex vivo I/R injury with or without IPC and found that IPC still protected these hearts, suggesting that cardiomyocyte ROMK does not participate significantly in the cardioprotective pathway elicited by IPC. Collectively, our findings from these novel strains of mice suggest that cardiomyocyte ROMK is not a central mediator of mitoKATP function, although it can affect mPTP activation threshold.
KW - Bartter's syndrome
KW - Ischemic preconditioning
KW - Kcnj1 or Kir1.1 or ROMK
KW - Mitochondrial ATP-sensitive potassium channel
KW - Mitochondrial permeability transition pore
KW - Renal potassium channel
UR - http://www.scopus.com/inward/record.url?scp=85078937724&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85078937724&partnerID=8YFLogxK
U2 - 10.1016/j.yjmcc.2020.01.010
DO - 10.1016/j.yjmcc.2020.01.010
M3 - Article
C2 - 32004507
AN - SCOPUS:85078937724
SN - 0022-2828
VL - 139
SP - 176
EP - 189
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
ER -