TY - JOUR
T1 - Global characterization of differential gene expression profiles in mouse Vγ1+ and Vγ4+ γδ T cells
AU - Dong, Peng
AU - Zhang, Siya
AU - Cai, Menghua
AU - Kang, Ning
AU - Hu, Yu
AU - Cui, Lianxian
AU - Zhang, Jianmin
AU - He, Wei
PY - 2014
Y1 - 2014
N2 - Peripheral γδ T cells in mice are classified into two major subpopulations, Vγ1+ and Vγ4+, based on the composition of T cell receptors. However, their intrinsic differences remain unclear. In this study, we analyzed gene expression profiles of the two subsets using Illumina HiSeq 2000 Sequencer. We identified 1995 transcripts related to the activation of Vγ1+ γδ T cells, and 2158 transcripts related to the activation of Vγ4+ γδ T cells. We identified 24 transcripts differentially expressed between the two subsets in resting condition, and 20 after PMA/Ionomycin treatment. We found that both cell types maintained phenotypes producing IFN- γ,TNF-α, TGF-β and IL-10. However, Vγ1+ γδ T cells produced more Th2 type cytokines, such as IL-4 and IL-5, while Vγ4+, γδ T cells preferentially produced IL-17. Our study provides a comprehensive gene expression profile of mouse peripheral Vγ1+ and Vγ4+, γδ T cells that describes the inherent differences between them.
AB - Peripheral γδ T cells in mice are classified into two major subpopulations, Vγ1+ and Vγ4+, based on the composition of T cell receptors. However, their intrinsic differences remain unclear. In this study, we analyzed gene expression profiles of the two subsets using Illumina HiSeq 2000 Sequencer. We identified 1995 transcripts related to the activation of Vγ1+ γδ T cells, and 2158 transcripts related to the activation of Vγ4+ γδ T cells. We identified 24 transcripts differentially expressed between the two subsets in resting condition, and 20 after PMA/Ionomycin treatment. We found that both cell types maintained phenotypes producing IFN- γ,TNF-α, TGF-β and IL-10. However, Vγ1+ γδ T cells produced more Th2 type cytokines, such as IL-4 and IL-5, while Vγ4+, γδ T cells preferentially produced IL-17. Our study provides a comprehensive gene expression profile of mouse peripheral Vγ1+ and Vγ4+, γδ T cells that describes the inherent differences between them.
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U2 - 10.1371/journal.pone.0112964
DO - 10.1371/journal.pone.0112964
M3 - Article
C2 - 25405356
AN - SCOPUS:84913549204
SN - 1932-6203
VL - 9
JO - PLoS One
JF - PLoS One
IS - 11
M1 - e112964
ER -