TY - JOUR
T1 - Genotyping of 75 SNPs using arrays for individual identification in five population groups
AU - Hwa, Hsiao Lin
AU - Wu, Lawrence Shih Hsin
AU - Lin, Chun Yen
AU - Huang, Tsun Ying
AU - Yin, Hsiang I.
AU - Tseng, Li Hui
AU - Lee, James Chun I
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Single nucleotide polymorphism (SNP) typing offers promise to forensic genetics. Various strategies and panels for analyzing SNP markers for individual identification have been published. However, the best panels with fewer identity SNPs for all major population groups are still under discussion. This study aimed to find more autosomal SNPs with high heterozygosity for individual identification among Asian populations. Ninety-six autosomal SNPs of 502 DNA samples from unrelated individuals of five population groups (208 Taiwanese Han, 83 Filipinos, 62 Thais, 69 Indonesians, and 80 individuals with European, Near Eastern, or South Asian ancestry) were analyzed using arrays in an initial screening, and 75 SNPs (group A, 46 newly selected SNPs; groups B, 29 SNPs based on a previous SNP panel) were selected for further statistical analyses. Some SNPs with high heterozygosity from Asian populations were identified. The combined random match probability of the best 40 and 45 SNPs was between 3.16 × 10−17 and 7.75 × 10−17 and between 2.33 × 10−19 and 7.00 × 10−19, respectively, in all five populations. These loci offer comparable power to short tandem repeats (STRs) for routine forensic profiling. In this study, we demonstrated the population genetic characteristics and forensic parameters of 75 SNPs with high heterozygosity from five population groups. This SNPs panel can provide valuable genotypic information and can be helpful in forensic casework for individual identification among these populations.
AB - Single nucleotide polymorphism (SNP) typing offers promise to forensic genetics. Various strategies and panels for analyzing SNP markers for individual identification have been published. However, the best panels with fewer identity SNPs for all major population groups are still under discussion. This study aimed to find more autosomal SNPs with high heterozygosity for individual identification among Asian populations. Ninety-six autosomal SNPs of 502 DNA samples from unrelated individuals of five population groups (208 Taiwanese Han, 83 Filipinos, 62 Thais, 69 Indonesians, and 80 individuals with European, Near Eastern, or South Asian ancestry) were analyzed using arrays in an initial screening, and 75 SNPs (group A, 46 newly selected SNPs; groups B, 29 SNPs based on a previous SNP panel) were selected for further statistical analyses. Some SNPs with high heterozygosity from Asian populations were identified. The combined random match probability of the best 40 and 45 SNPs was between 3.16 × 10−17 and 7.75 × 10−17 and between 2.33 × 10−19 and 7.00 × 10−19, respectively, in all five populations. These loci offer comparable power to short tandem repeats (STRs) for routine forensic profiling. In this study, we demonstrated the population genetic characteristics and forensic parameters of 75 SNPs with high heterozygosity from five population groups. This SNPs panel can provide valuable genotypic information and can be helpful in forensic casework for individual identification among these populations.
KW - Array
KW - Forensic genetics
KW - Individual identification
KW - Random match probability
KW - Single nucleotide polymorphism
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U2 - 10.1007/s00414-015-1250-y
DO - 10.1007/s00414-015-1250-y
M3 - Article
C2 - 26297200
AN - SCOPUS:84954394473
SN - 0937-9827
VL - 130
SP - 81
EP - 89
JO - International Journal of Legal Medicine
JF - International Journal of Legal Medicine
IS - 1
ER -