TY - JOUR
T1 - Genotypic and phenotypic characterization of human immunodeficiency virus type 1 isolates circulating in pregnant women from Mozambique
AU - Abreu, Celina Monteiro
AU - Brindeiro, Patrícia A.
AU - Martins, Angélica N.
AU - Arruda, Mônica B.
AU - Bule, Emilio
AU - Stakteas, Sergio
AU - Tanuri, Amilcar
AU - De Moraes Brindeiro, Rodrigo
N1 - Funding Information:
This work was supported by CNPq PROAFRICA (Proc# 491367/2005-8) with Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. We are thankful to Dr. Alfredo Vergara, CDC Mozambique, Global AIDS Program for the helpful discussion of the data presented here.
PY - 2008/11
Y1 - 2008/11
N2 - This work evaluated HIV-1 subtypes from different geographic regions and phenotypic data from drug-naïve HIV-positive pregnant women from Mozambique. We analyzed 75 pol sequences from patients and the distribution of the subtypes in three regions of Mozambique and found that the majority of samples analyzed clustered with subtype C. In the northern region, multiple variants were found 5 (~18%) subtype A, 3 (~11%) subtype D and 2 (~7.1%) mosaics (A/C/D and C/D), whereas 18 (64.3%) isolates were subtype C, from a total of 28 samples. Already in the southern region, only one (5%) isolate of 20 samples was subtype D, and the other 19 (95%) isolates were subtype C. All 27 (100%) isolates from the central region grouped within clade C. No primary resistance mutations to IP, NNRTI or NRTI were found. There was no evidence of phenotypic resistance in any of the isolates tested, suggesting that neither the polymorphism in the protease, nor the one found at codon 215 of the RT gene caused an increase in phenotypic resistance. This finding suggests that HAART regimens indicated by WHO will probably be successful in Mozambique.
AB - This work evaluated HIV-1 subtypes from different geographic regions and phenotypic data from drug-naïve HIV-positive pregnant women from Mozambique. We analyzed 75 pol sequences from patients and the distribution of the subtypes in three regions of Mozambique and found that the majority of samples analyzed clustered with subtype C. In the northern region, multiple variants were found 5 (~18%) subtype A, 3 (~11%) subtype D and 2 (~7.1%) mosaics (A/C/D and C/D), whereas 18 (64.3%) isolates were subtype C, from a total of 28 samples. Already in the southern region, only one (5%) isolate of 20 samples was subtype D, and the other 19 (95%) isolates were subtype C. All 27 (100%) isolates from the central region grouped within clade C. No primary resistance mutations to IP, NNRTI or NRTI were found. There was no evidence of phenotypic resistance in any of the isolates tested, suggesting that neither the polymorphism in the protease, nor the one found at codon 215 of the RT gene caused an increase in phenotypic resistance. This finding suggests that HAART regimens indicated by WHO will probably be successful in Mozambique.
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U2 - 10.1007/s00705-008-0215-6
DO - 10.1007/s00705-008-0215-6
M3 - Article
C2 - 18839058
AN - SCOPUS:55949133985
SN - 0304-8608
VL - 153
SP - 2013
EP - 2017
JO - Archives of Virology
JF - Archives of Virology
IS - 11
ER -