Genome-wide functional analysis of pathogenicity genes in the rice blast fungus

Junhyun Jeon; Sook Young Park; Myoung Hwan Chi; Jaehyuk Choi; Jongsun Park; Hee Sool Rho; Soonok Kim; Jaeduk Goh; Sungyong Yoo; Jinhee Choi; Ju Young Park; Mihwa Yi; Seonyoung Yang; Min Jung Kwon; Seong Sook Han; Byeong Ryun Kim; Chang Hyun Khang; Bongsoo Park; Se Eun Lim; Kyongyong Jung; Sunghyung Kong; Maruthachalam Karunakaran; Hong Sik Oh; Hyojeong Kim; Seryun Kim; Jaejin Park; Soyoung Kang; Woo Bong Choi; Seogchan Kang; Yong Hwan Lee

doi:10.1038/ng2002

Genome-wide functional analysis of pathogenicity genes in the rice blast fungus

Junhyun Jeon, Sook Young Park, Myoung Hwan Chi, Jaehyuk Choi, Jongsun Park, Hee Sool Rho, Soonok Kim, Jaeduk Goh, Sungyong Yoo, Jinhee Choi, Ju Young Park, Mihwa Yi, Seonyoung Yang, Min Jung Kwon, Seong Sook Han, Byeong Ryun Kim, Chang Hyun Khang, Bongsoo Park, Se Eun Lim, Kyongyong JungSunghyung Kong, Maruthachalam Karunakaran, Hong Sik Oh, Hyojeong Kim, Seryun Kim, Jaejin Park, Soyoung Kang, Woo Bong Choi, Seogchan Kang, Yong Hwan Lee

Research output: Contribution to journal › Article › peer-review

170 Scopus citations

Abstract

Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.

Original language	English (US)
Pages (from-to)	561-565
Number of pages	5
Journal	Nature genetics
Volume	39
Issue number	4
DOIs	https://doi.org/10.1038/ng2002
State	Published - Apr 2007
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1038/ng2002

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Jeon, J., Park, S. Y., Chi, M. H., Choi, J., Park, J., Rho, H. S., Kim, S., Goh, J., Yoo, S., Choi, J., Park, J. Y., Yi, M., Yang, S., Kwon, M. J., Han, S. S., Kim, B. R., Khang, C. H., Park, B., Lim, S. E., ... Lee, Y. H. (2007). Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. Nature genetics, 39(4), 561-565. https://doi.org/10.1038/ng2002

Jeon, J, Park, SY, Chi, MH, Choi, J, Park, J, Rho, HS, Kim, S, Goh, J, Yoo, S, Choi, J, Park, JY, Yi, M, Yang, S, Kwon, MJ, Han, SS, Kim, BR, Khang, CH, Park, B, Lim, SE, Jung, K, Kong, S, Karunakaran, M, Oh, HS, Kim, H, Kim, S, Park, J, Kang, S, Choi, WB, Kang, S & Lee, YH 2007, 'Genome-wide functional analysis of pathogenicity genes in the rice blast fungus', Nature genetics, vol. 39, no. 4, pp. 561-565. https://doi.org/10.1038/ng2002

@article{52bd82d070654bca8713e21bcf01684f,

title = "Genome-wide functional analysis of pathogenicity genes in the rice blast fungus",

abstract = "Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.",

author = "Junhyun Jeon and Park, {Sook Young} and Chi, {Myoung Hwan} and Jaehyuk Choi and Jongsun Park and Rho, {Hee Sool} and Soonok Kim and Jaeduk Goh and Sungyong Yoo and Jinhee Choi and Park, {Ju Young} and Mihwa Yi and Seonyoung Yang and Kwon, {Min Jung} and Han, {Seong Sook} and Kim, {Byeong Ryun} and Khang, {Chang Hyun} and Bongsoo Park and Lim, {Se Eun} and Kyongyong Jung and Sunghyung Kong and Maruthachalam Karunakaran and Oh, {Hong Sik} and Hyojeong Kim and Seryun Kim and Jaejin Park and Soyoung Kang and Choi, {Woo Bong} and Seogchan Kang and Lee, {Yong Hwan}",

note = "Funding Information: We are grateful to K. Lee and N.J. Talbot for their comments and suggestions on the manuscript. This research was partially supported by a grant from the Biogreen21 project funded by the Rural Development Administration, by grants from the Crop Functional Genomics Center (CG1421) and the Microbial Genomics and Applications Center (0462-20060021) of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, and by Korean Research Foundation Grant (KRF-2004-005-F00013) to Y.H.L. Requests for materials should be addressed to Y.H.L. (yonglee@snu.ac.kr).",

year = "2007",

month = apr,

doi = "10.1038/ng2002",

language = "English (US)",

volume = "39",

pages = "561--565",

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AU - Jeon, Junhyun

AU - Park, Sook Young

AU - Chi, Myoung Hwan

AU - Choi, Jaehyuk

AU - Park, Jongsun

AU - Rho, Hee Sool

AU - Kim, Soonok

AU - Goh, Jaeduk

AU - Yoo, Sungyong

AU - Choi, Jinhee

AU - Park, Ju Young

AU - Yi, Mihwa

AU - Yang, Seonyoung

AU - Kwon, Min Jung

AU - Han, Seong Sook

AU - Kim, Byeong Ryun

AU - Khang, Chang Hyun

AU - Park, Bongsoo

AU - Lim, Se Eun

AU - Jung, Kyongyong

AU - Kong, Sunghyung

AU - Karunakaran, Maruthachalam

AU - Oh, Hong Sik

AU - Kim, Hyojeong

AU - Kim, Seryun

AU - Park, Jaejin

AU - Kang, Soyoung

AU - Choi, Woo Bong

AU - Kang, Seogchan

AU - Lee, Yong Hwan

N1 - Funding Information: We are grateful to K. Lee and N.J. Talbot for their comments and suggestions on the manuscript. This research was partially supported by a grant from the Biogreen21 project funded by the Rural Development Administration, by grants from the Crop Functional Genomics Center (CG1421) and the Microbial Genomics and Applications Center (0462-20060021) of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, and by Korean Research Foundation Grant (KRF-2004-005-F00013) to Y.H.L. Requests for materials should be addressed to Y.H.L. (yonglee@snu.ac.kr).

PY - 2007/4

Y1 - 2007/4

N2 - Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.

AB - Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.

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ER -

Genome-wide functional analysis of pathogenicity genes in the rice blast fungus

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