TY - JOUR
T1 - Genome sequencing and analysis of geographically diverse clinical isolates of herpes simplex virus 2
AU - Newman, Ruchi M.
AU - Lamers, Susanna L.
AU - Weiner, Brian
AU - Ray, Stuart C.
AU - Colgrove, Robert C.
AU - Diaz, Fernando
AU - Jing, Lichen
AU - Wang, Kening
AU - Saif, Sakina
AU - Young, Sarah
AU - Henn, Matthew
AU - Laeyendecker, Oliver
AU - Tobian, Aaron A.R.
AU - Cohen, Jeffrey I.
AU - Koelle, David M.
AU - Quinn, Thomas C.
AU - Knipe, David M.
N1 - Publisher Copyright:
© 2015, American Society for Microbiology.
PY - 2015
Y1 - 2015
N2 - Herpes simplex virus 2 (HSV-2), the principal causative agent of recurrent genital herpes, is a highly prevalent viral infection worldwide. Limited information is available on the amount of genomic DNA variation between HSV-2 strains because only two genomes have been determined, the HG52 laboratory strain and the newly sequenced SD90e low-passage-number clinical isolate strain, each from a different geographical area. In this study, we report the nearly complete genome sequences of 34 HSV-2 lowpassage- number and laboratory strains, 14 of which were collected in Uganda, 1 in South Africa, 11 in the United States, and 8 in Japan. Our analyses of these genomes demonstrated remarkable sequence conservation, regardless of geographic origin, with the maximum nucleotide divergence between strains being 0.4% across the genome. In contrast, prior studies indicated that HSV-1 genomes exhibit more sequence diversity, as well as geographical clustering. Additionally, unlike HSV-1, little viral recombination between HSV-2 strains could be substantiated. These results are interpreted in light of HSV-2 evolution, epidemiology, and pathogenesis. Finally, the newly generated sequences more closely resemble the low-passage-number SD90e than HG52, supporting the use of the former as the new reference genome of HSV-2.
AB - Herpes simplex virus 2 (HSV-2), the principal causative agent of recurrent genital herpes, is a highly prevalent viral infection worldwide. Limited information is available on the amount of genomic DNA variation between HSV-2 strains because only two genomes have been determined, the HG52 laboratory strain and the newly sequenced SD90e low-passage-number clinical isolate strain, each from a different geographical area. In this study, we report the nearly complete genome sequences of 34 HSV-2 lowpassage- number and laboratory strains, 14 of which were collected in Uganda, 1 in South Africa, 11 in the United States, and 8 in Japan. Our analyses of these genomes demonstrated remarkable sequence conservation, regardless of geographic origin, with the maximum nucleotide divergence between strains being 0.4% across the genome. In contrast, prior studies indicated that HSV-1 genomes exhibit more sequence diversity, as well as geographical clustering. Additionally, unlike HSV-1, little viral recombination between HSV-2 strains could be substantiated. These results are interpreted in light of HSV-2 evolution, epidemiology, and pathogenesis. Finally, the newly generated sequences more closely resemble the low-passage-number SD90e than HG52, supporting the use of the former as the new reference genome of HSV-2.
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U2 - 10.1128/JVI.01303-15
DO - 10.1128/JVI.01303-15
M3 - Article
C2 - 26018166
AN - SCOPUS:84938066521
SN - 0022-538X
VL - 89
SP - 8219
EP - 8232
JO - Journal of virology
JF - Journal of virology
IS - 16
ER -